B0274
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
tablet
Manufacturer: Sigma Aldrich
CAS Number: 6578-06-9
Synonym(S): BCIP® p-toluidine salt, X-phosphate p-toluidine salt
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 10 TABS | B0274-10-TABS | In Stock | ₹ 40,734.48 |
| 25 TABS | B0274-25-TABS | In Stock | ₹ 81,035.95 |
B0274 - 10 TABS
In Stock
Quantity
1
Base Price: ₹ 40,734.48
GST (18%): ₹ 7,332.206
Total Price: ₹ 48,066.686
form
tablet
Quality Level
200
storage temp.
−20°C
SMILES string
Cc1ccc(N)cc1.OP(O)(=O)Oc2c[nH]c3ccc(Br)c(Cl)c23
InChI
1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3
InChI key
QEIFSLUFHRCVQL-UHFFFAOYSA-N
Other Options
Related Products
Description
- Specificity: Substrate of choice for use with alkaline phosphatase in immunoblotting and, less commonly, in immunohistological staining procedures. High assay sensitivity is achieved via amplification when BCIP is used in conjunction with Nitro Blue Tetrazolium (NBT) Tablets (Catalog No. N5514). This substrate produces an insoluble end product that is blue-purple in color and can be observed visually.
- Application: For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
- Quantity: Contains 25 mg substrate per tablet.
- Substrates: Histochemical substrate for alkaline phosphatase.
- Legal Information: BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
SAFETY INFORMATION
WGK
WGK 3
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Compare Similar Items
Show Difference
form: tablet
Quality Level: 200
storage temp.: −20°C
SMILES string: Cc1ccc(N)cc1.OP(O)(=O)Oc2c[nH]c3ccc(Br)c(Cl)c23
InChI: 1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3
InChI key: QEIFSLUFHRCVQL-UHFFFAOYSA-N
form:
tablet
Quality Level:
200
storage temp.:
−20°C
SMILES string:
Cc1ccc(N)cc1.OP(O)(=O)Oc2c[nH]c3ccc(Br)c(Cl)c23
InChI:
1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3
InChI key:
QEIFSLUFHRCVQL-UHFFFAOYSA-N
form: liquid
Quality Level: 300
storage temp.: 2-8°C
SMILES string: __
InChI: __
InChI key: __
form:
liquid
Quality Level:
300
storage temp.:
2-8°C
SMILES string:
__
InChI:
__
InChI key:
__
form: powder
Quality Level: 200
storage temp.: 2-8°C
SMILES string: [H][C@]12CS[C@@H](CCCCC(O)=O)[C@@]1([H])NC(=O)N2
InChI: __
InChI key: __
form:
powder
Quality Level:
200
storage temp.:
2-8°C
SMILES string:
[H][C@]12CS[C@@H](CCCCC(O)=O)[C@@]1([H])NC(=O)N2
InChI:
__
InChI key:
__
form: powder
Quality Level: 200
storage temp.: 2-8°C
SMILES string: [H][C@]12CS[C@@H](CCCCC(O)=O)[C@@]1([H])NC(=O)N2
InChI: __
InChI key: __
form:
powder
Quality Level:
200
storage temp.:
2-8°C
SMILES string:
[H][C@]12CS[C@@H](CCCCC(O)=O)[C@@]1([H])NC(=O)N2
InChI:
__
InChI key:
__