Description

• General description: α-Chymotrypsin from bovine pancreas (bovine pancreatic α-chymotrypsin, CHT) is an enzyme protein.[1] The influence of varying concentrations of organic solvents like ethanol, 1,4-dioxane and acetonitrile on CHT has been reported.[2]
• Application: α-Chymotrypsin from bovine pancreas is used for the following applications:Protein Identification by mass spectrometry (MS)[4]The isolation and characterization of myosin heavy chains[5]Toxin preparation[6]The incubation of infected and uninfected cells for analysis of cellular proteins by SDS-PAGE[7]α-Chymotrypsin from bovine pancreas (BPC) may be used as a catalyst in the preparation of tetrahydroquinoline derivatives by Povarov reaction.[8]
• Biochem/physiol Actions: α-Chymotrypsin from bovine pancreas is stabilized by Ca2+ and catalyzes the hydrolysis of peptide bonds in particular the amino acids tyrosine, phenylalanine, tryptophan, and leucine at their C-terminal side.[9] α-Chymotrypsin is inhibited by Cu2+ and Hg.[9]
• Unit Definition: One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.
• Other Notes: View more information on chymotrypsin at www.sigma-aldrich.com/enzymeexplorer