Invitrogen™ Fluo-5N, Pentapotassium Salt, cell impermeant

Description

• Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca 2+
• Cells may be physically loaded with cell-impermeant salt forms of these indicators using patch pipet or microinjection or Influx™ pinocytotic cell-loading reagent Compatible with excitation at 488nm by argon-ion laser sources, making them useful for confocal microscopy, flow cytometry, and microplate screening applications Exhibit fluorescence increase upon binding Ca 2+ with little shift in wavelength BAPTA-based indicators bind various heavy metal cations (e.g., Mn 2+ , Zn 2+ , Pb 2+ ) with substantially higher affinity than Ca 2+ Perturbations to calcium measurements caused by presence of the ions can be controlled using heavy metal-selective chelator Label (Ex/Em of Ca 2+ -bound form): Fluo-5N (494/516nm) Fluorescence intensity increase upon binding Ca 2+ : >100 fold K d for Ca 2+ in buffer: approximately 90μM Calcium Detection, Cell Analysis, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling Order Info Shipping Condition: Room temperature