Invitrogen™ Competent Cell Sampler

Description

• The Competent Cell Sampler Kit contains a pair of four different strains of chemically competent E
• coli cells in One Shot format
• Each single-use vial of cells is ready to use, with no extra pipetting steps or freeze-thaw cycles that reduce transformation efficiency
• Use this kit to evaluate which strains best suit the different types of DNA used in your lab
• One Shot Mach1 T1 Chemically Competent E
• coli One Shot Mach1 T1 Chemically Competent E
• coli cells are phage resistant and have a faster doubling time (∼50 minutes) than other popular E
• coli cells
• As a result, Mach1-T1 R colonies are clearly visible within eight hours of plating transformation on ampicillin selection plates
• From an overnight colony, minipreps can be performed after four hours of growth
• The Mach1-T1 R strain is derived from a non-K-12, wild-type W E
• coli strain that has high growth speed and has been genetically manipulated to possess the most useful cloning strain features
• Mach1-T1 R competent E
• coli cells come in One Shot format (Cat
• No
• C862003) or MultiShot StripWell and FlexPlate formats for high throughput applications (Cat
• No
• C869601 and C8681201)
• One Shot OmniMAX2 T1 R Chemically Competent E
• coli The One Shot OmniMAX2 T1 R Chemically Competent E
• coli strain is a versatile phage-resistant strain, perfect to use in all cloning applications, including Gateway technology, TOPO PCR cloning, and cDNA and gDNA library construction
• It offers the highest transformation efficiency in a chemically competent One Shot format with 5 x 10 9 transformants/μg plasmid DNA
• The ccdA gene present on the F´ episome, carried by OmniMAX 2-T1 R , is deleted, allowing negative selection of Gateway vectors since the strain is sensitive to the ccdB gene product
• OmniMAX 2-T1 R cells are offered in our One Shot format (Cat
• No
• C854003) or MultiShot FlexPlate format for high throughput applications (Cat
• No
• C8581201)
• One Shot Stbl3 Chemically Competent E
• coli One Shot Stbl3 Chemically Competent E
• coli are designed for cloning direct repeats found in lentiviral expression vectors as these cells reduce the frequency of homologous recombination of direct repeats found in lentiviral or retroviral vectors
• Stbl3 had been essential for many applications, including genome editing CRISPR workflows or vaccine development
• The DNA yield from Stbl3 cells is often >10-fold higher than standard cloning strains
• Stbl3 chemically competent cells are available in One Shot format (Cat
• No
• C737303) or MultiShot StripWell and FlexPlate formats (Cat
• No
• C739601 and C7381201) for high throughput applications
• One Shot TOP10 Chemically Competent E
• coli One Shot TOP10 Chemically Competent E
• coli are one of the most popular competent cell strains and are perfect for a broad range of cloning applications
• They are included with many of our cloning and expression kits like TOPO and Gateway cloning kits and complement our GeneArt Gene Synthesis Services
• One Shot TOP10 competent E
• coli have a transformation efficiency of 1 x 10 9 cfu/μg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation
• They allow stable replication of high-copy number plasmids
• TOP10 chemically competent cells are available in the One Shot format (Cat
• No
• C404003) or MultiShot StripWell and FlexPlate formats (Cat
• No
• C409601 and C4081201) for high throughput applications
• TOP10 cells are also available for transformation using electroporation (Cat
• No
• C404050)
• Order Info Shipping Condition: Dry Ice