Thermo Scientific™ Jump-In™ GripTite™ HEK 293 Kit

Description

• The high retargeting efficiency, made possible by the R4 sites in the GripTite™ HEK293 cell line, allows the use of the isogenic pool for additional experiments without the need for clonal selection
• Alternatively, the high retargeting efficiency allows for the easy selection of a positive stable clone expressing your gene of interest
• The Jump-In™ GripTite™ HEK293 Kit Lets You: • Quickly and efficiently develop stably engineered isogenic cell pools in about half the time compared to traditional cell engineering methods • Utilize isogenic expression from a defined genomic locus as the ideal solution for comparative analysis of gene families, isoforms, or orthologs • Generate multiple cell lines in parallel using the simplified work flow • Easily access the technology without complicated licenses or restrictions to interpret Save Time with Rapid and Efficient Generation of Engineered Cell Lines With the Jump-In™ GripTite™ HEK293 Kit you can generate functional cell pools in as little as 2 weeks without laborious clone isolation and analysis, and the streamlined protocol makes it easier to generate several cell lines at the same time
• Even generation of clonal cell lines can be done with reduced time and effort due to the high percentage of positive clones
• In addition, the Jump-In™ technology gives you the freedom to generate an unlimited number of cell lines without restrictive licensing requirements
• Expand Your Experimental Capabilities The Jump-In™GripTite™ HEK293 Kit is the ideal solution for cells and assays where transient engineering technologies are problematic, as well as for difficult to 'engineer' cell lines
• The kit also provides a convenient way to create target panels of gene families, isoforms, or orthologs
• Genes coding for large proteins or multi-unit proteins are not a problem since the Gateway™ destination vectors accept large inserts
• The kit includes: • pJTI™ R4 Dest CMV pA vector (100 μg) • pJTI™ R4 Int vector (100 μg) • Jump-In™GripTite™ HEK293 Cells (2 vials @ 1 ml each) For Research Use Only
• Not intended for any animal or human therapeutic or diagnostic use.