A24229
Invitrogen™ MAX Efficiency™ Transformation Reagent for Algae
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | A24229-Each-of-1 | In Stock | ₹ 18,156.00 |
A24229 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 18,156.00
GST (18%): ₹ 3,268.08
Total Price: ₹ 21,424.08
Cell Line
Chlamydomonas
Expression System
Algae
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Product Line
GeneArt™
Selection Agent (Eukaryotic)
Hygromycin
Content And Storage
Contains:1 bottle Transformation Reagent, 250 mL
For Use With (Application)
Gel Electrophoresis
No. of Reactions
40 Reactions
Product Type
Transformation Reagent
Related Products
Description
- MAX Efficiency™ Transformation Reagent for Algae is a buffer that enhances transformation efficiency for multiple strains of Chlamydomonas species
- One of the biggest hurdles in research and development with Chlamydomonas has been the introduction of exogenous DNA into Chlamydomonas strains due to rigid cell walls
- Methods such as glass bead agitation, electroporation, and microparticle bombardment are available but often result in low transformation efficiency
- MAX Efficiency™ Transformation Reagent for Algae increases the permeability of the Chlamydomonas cell wall and facilitates increased delivery of DNA into the cell nucleus by electroporation
- Simply grow the Chlamydomonas cells to early log phase, harvest by centrifugation, and wash twice with MAX Efficiency™ Transformation Reagent prior to electroporation
- To date, we have seen >200-fold increase in transformation efficiency over previously recommended conditions in 10 different Chlamydomonas strains, including wild type and mutants, using circular or linear DNA, as well as PCR fragments
- Obtain >1000 transformants/μg DNA for cell wall(+) strains Obtain >100 transformants/μg DNA for cell wall(-) strains Transform circular or linear DNA or PCR fragments For Research Use Only
- Not for use in diagnostic procedures.
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Cell Line: Chlamydomonas
Expression System: Algae
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Product Line: GeneArt™
Selection Agent (Eukaryotic): Hygromycin
Content And Storage: Contains:1 bottle Transformation Reagent, 250 mL
For Use With (Application): Gel Electrophoresis
No. of Reactions: 40 Reactions
Product Type: Transformation Reagent
Cell Line:
Chlamydomonas
Expression System:
Algae
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Product Line:
GeneArt™
Selection Agent (Eukaryotic):
Hygromycin
Content And Storage:
Contains:1 bottle Transformation Reagent, 250 mL
For Use With (Application):
Gel Electrophoresis
No. of Reactions:
40 Reactions
Product Type:
Transformation Reagent
Cell Line: __
Expression System: __
High-throughput Compatibility: __
Product Line: __
Selection Agent (Eukaryotic): __
Content And Storage: __
For Use With (Application): __
No. of Reactions: __
Product Type: __
Cell Line:
__
Expression System:
__
High-throughput Compatibility:
__
Product Line:
__
Selection Agent (Eukaryotic):
__
Content And Storage:
__
For Use With (Application):
__
No. of Reactions:
__
Product Type:
__
Cell Line: __
Expression System: __
High-throughput Compatibility: __
Product Line: __
Selection Agent (Eukaryotic): __
Content And Storage: __
For Use With (Application): __
No. of Reactions: __
Product Type: __
Cell Line:
__
Expression System:
__
High-throughput Compatibility:
__
Product Line:
__
Selection Agent (Eukaryotic):
__
Content And Storage:
__
For Use With (Application):
__
No. of Reactions:
__
Product Type:
__
Cell Line: __
Expression System: __
High-throughput Compatibility: __
Product Line: __
Selection Agent (Eukaryotic): __
Content And Storage: Contains:• 1 bottle Cell Lysis Buffer• 1 tube Proteinase K• 1 tube PCR Supermix• 1 tube Water• 1 tube T7E1 Detection Enzyme• 1 tube T7E1 Detection Reaction Buffer• 1 tube Control Template & PrimersStore all components at -5° to -30°C.
For Use With (Application): __
No. of Reactions: 20 Reactions
Product Type: Genomic Cleavage Detection Kit
Cell Line:
__
Expression System:
__
High-throughput Compatibility:
__
Product Line:
__
Selection Agent (Eukaryotic):
__
Content And Storage:
Contains:• 1 bottle Cell Lysis Buffer• 1 tube Proteinase K• 1 tube PCR Supermix• 1 tube Water• 1 tube T7E1 Detection Enzyme• 1 tube T7E1 Detection Reaction Buffer• 1 tube Control Template & PrimersStore all components at -5° to -30°C.
For Use With (Application):
__
No. of Reactions:
20 Reactions
Product Type:
Genomic Cleavage Detection Kit