Thermo Scientific™ TransformAid Bacterial Transformation Kit

Description

• Employ a novel method for rapid preparation of chemically-competent E
• coli cells from overnight bacterial cultures or bacterial colonies with the Thermo Scientific TransformAid™ Bacterial Transformation Kit
• The key component of this system is the unique T-solution, which produces competent cells in a few easy steps
• The quick and convenient procedure guarantees transformation efficiencies of more than 107 transformants per μg of plasmid DNA, ideal for routine cloning experiments
• The option to use bacterial colonies for preparation of competent cells adds flexibility to any cloning experiment
• The TransformAid Bacterial Transformation Kit can be used with most E
• coli strains commonly used for cloning: JM107, JM109, XL1-Blue, SURE, TOPP2, W3110, NM527, AD494, CJ236, GM2163, C600, BL21, M15, NM522, HB101, ER2267
• Highlights Efficient – more than 107 transformants per μg of plasmid DNA Practical – E
• coli competent cells can be prepared from an overnight culture or from bacterial colonies Fast – less than 1 hour from the overnight bacterial culture to cell plating
• Starting from bacterial colonies, the procedure requires only 2.5 hours Easy – all procedures are performed on ice on your bench
• Brief centrifugations are carried out in regular microcentrifuges Applications Routine cloning experiments Blunt-end cloning TA cloning Includes C-Medium T-Solution (A) T-Solution (B) Detailed Protocol Note Competent cells prepared with TransformAid Bacterial Transformation Kit are suitable for direct-use only
• Freezing down and storage at -70°C is not recommended.