Invitrogen™ Human Estrogen Receptor alpha (aa 247-261) Synthetic Peptide

Description

• Estrogen Receptor A (ERa) (residues 247-261) Synthetic Peptide, PEP-013, from Invitrogen
• Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors
• The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA
• When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins
• There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively)
• Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa))
• Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function
• They also play a role in other tissues such as bone
• Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis
• PEP-013 is a 15 amino acid (amino acids 247-261) synthetic peptide whose sequence is from the ER-DNA binding domain of the estrogen receptor a
• The sequence of this peptide is (amino to carboxy terminus): E-V-G-M-M-K-G-G-I-R-K-D-R-R-GThis peptide may be used for neutralization and control experiments with the monoclonal antibody that reacts with this product and estrogen receptor a, catalog &Num MA1-310
• Using a solution with equal weights per unit volume of peptide and corresponding antibody will yield a solution with a large molar excess of peptide that is able to competitively bind the antibody.Reconstitute with 0.1 mL of distilled water.