Invitrogen™ Human CREB (aa 123-136) Synthetic Peptide

Description

• Human CREB (residues 123-136) Synthetic Peptide, PEP-142, from Invitrogen
• CREB (Cyclic AMP response element binding protein) is a 43 kDa basic/leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors
• Activation of CREB is dependent upon the phosphorylation of serine 133
• Phosphorylation of CREB occurs via p44/42 MAP kinase and p90RSK, and via p38 MAP kinase and MSK1
• Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor
• CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133
• CREB is involved in different cellular processes including the synchronization of circadian rhythmicity, differentiation of adipose cells, and learning and memory
• In humans, the gene is located on the q arm of chromosome 2
• Diseases associated with CREB dysfunction include Alzheimer’s Disease, histiocytoma and soft tissue melanoma
• PEP-142 is a 14 amino acid synthetic peptide whose sequence corresponds to residues 123-136 of human CREB
• The sequence of this peptide is (amino to carboxy terminus): K (123)-R-R-E-I-L-S-R-R-P-S-Y-R-K (136)This peptide may be used for neutralization and control experiments with the polyclonal antibody that reacts with this product and human CREB, catalog &Num PA1-850
• Using a solution of peptide of equal volume and concentration to the corresponding antibody will yield a large molar excess of peptide (∽70-fold) for competitive inhibition of antibody-protein binding reactions.Reconstitute with 0.1 mL of distilled water.