Invitrogen™ Human Fibronectin Native Protein

Description

• Fibronectin (human) Native Protein, RP-43130, from Invitrogen
• Fibronectin is a disulfide-bonded dimer with a molecular weight range of 230-250 kDa
• In the extracellular matrix of several connective tissues and vessels, fibronectin is present as an insoluble protein that is extensively cross-linked by interchain disulfide bonds forming high molecular mass polymers
• Fibronectin is most abundant during embryonic development and tissue remodeling
• Fibronectin is also present at high concentrations as a soluble plasma protein
• Fibronectin is organized as a linear series of repeating modules which form domains for interaction with fibronectin itself, other matrix components (e.g
• collagen and heparin) and receptors on cells (e.g
• integrins)
• Fibronectin is present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix
• Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis
• Fibronectin has been implicated in carcinoma development in lung cancer
• Further, Fibronectin expression is increased especially in non-small cell lung carcinoma
• The adhesion of lung carcinoma cells to Fibronectin enhances tumorgenecity and confers resistance to apoptosis induced by standard chemotherapeutic agents
• The gene encoding Fibronectin has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants
• However, the full-length nature of some Fibronectin variants has not been determined
• RP-43130 contains native human Fibronectin protein and may be used as a positive control in many immunological assays
• The RP-43130 protein molecular weight is ∽550 kDa
• To reconstitute, completely equilibrate lyophilized protein to room temperature and add room temperature ultrapure water to protein, using 3.14 mL of water to achieve a final concentration of 0.65mg/mL
• Place in 37°C water bath to dissolve
• The product is of human blood/plasma origin
• Although the starting material was tested prior to initiation of the manufacturing process, and was found negative or nonreactive for anti-HIV-1/2, HIV-1 antigen(s), HBsAg, STS, anti-HCV, anti-HBcore and anti-HTLV I & II, extreme caution should be used when handling this material as there is a margin of error in all tests
• This product is to be used for IN VITRO research purposes only, and is not intended for clinical or diagnostic use
• Reconstitute with 1.36 milliliters of water to achieve 1.5mg/mL.