India Ink wet mounts are used for the visualization of encapsulated microorganisms, especially Cryptococcus neoformans
India Ink is added to each positive and negative suspension on a glass slide and observed under a microscope
The positive control provides an encapsulated suspension of Cryptococcus neoformans to be utilized when performing India Ink procedures The negative control provides a non-encapsulated suspension of Candida sp
to be utilized when performing India Ink EXPECTED RESULTS Positive: The polysaccharide capsules will exclude the particles of ink, and the capsules will appear as clear halos around the organisms on semiopaque backgrounds Negative: No capsules present, no clear halos observed