BD Fixable Viability Stain 570

Description

• Permeable plasma cell membranes, such as those present in necrotic cells, allow intracellular diffusion of dye and covalent binding to higher overall concentrations of amines than in non-permeable live cells Necrotic cells present in a typical in vitro assay label with higher levels of dye increases their fluorescence intensity 10-20 fold over viable cells Labeled cells can be fixed with formaldehyde for downstream decontamination, freezing and/or permeabilization and subsequent intracellular staining while maintaining stable viability stain fluorescence Excited by Yellow-Green laser (with an excitation maximum of 547nm), but is also well-excited by the Blue laser Fluorescence emission maximum of 573nm Applications: Flow cytometry, Intracellular staining