Thermo Scientific™ Methanol

Description

• Thermo Scientific™ Remel™ Absolute Methanol is used with the modified acid-fast stain procedure for detection of Cryptosporidium in clinical specimens
• Ma and Soave recommended a modified acid-fast staining procedure because of its simplicity and specificity for detecting Cryptosporidium 1
• Garcia recommended a more recent modification of the Kinyoun carbolfuchsin stain that is used to detect Cryptosporidium oocysts in clinical specimens 2,3
• Absolute Methanol is a fixative used to preserve the morphology of the cells and aids in detection of Cryptosporidium oocysts
• Cryptosporidium is an intestinal protozoan parasite well known for causing diarrhoea in animals and has more recently been recognized for causing human disease
• Oocysts are spherical 4-6μm in diameter, and stain pink to red to purple with Kinyoun carbolfuchsin stain
• Most will contain several dark staining granules
• Ready-to-use – Prepared reagents Easy-to-store – Store in its original container at room temperature (20-25°C) until further use Easy to interpret – Oocysts are spherical and stain pink to red to purple with Kinyoun carbolfuchsin stain whereas yeast cells which are morphologically like Cryptosporidium , stain blue or green depending on the counterstain used
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• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.