Thermo Scientific™ Phenolic Acridine Orange Decolorizer

Description

• Use Thermo Scientific™ Remel™ Phenolic Acridine Orange Decolorizer along with Phenolic Acridine Orange to detect acid-fast bacilli in clinical specimens
• In 1977, Kronvall and Myhre described the use of acridine orange stain to detect microorganisms in direct smears prepared from clinical specimens 1
• In 1955, Smithwick et al
• described an acid-fast method which incorporated an acridine orange solution that contained phenol 2
• Phenolic Acridine Orange stain was found to produce less nonspecific fluorescence of background material
• This makes it superior to auramine O
• Phenolic Acridine Orange is a stain which penetrates the mycobacterial cell wall and binds to the nucleic acid of bacteria and other cells, either in the native or the denatured state
• Methylene blue that has been added to Phenolic Acridine Orange decolorizer reduces background fluorescence in stained smears
• Ready-to-use – Liquid reagent Easy-to-observe – Examine smears with a fluorescent microscope with 200 X magnification and confirm the morphology with 600 X magnification
• Easy-to-interpret – Positive test shows bright-red to orange fluorescent bacilli with characteristic morphology confirmed at 600 X magnification
• In case of negative test no bright-red to orange fluorescent bacilli observed after examination of 30 fields at 200 X magnification
• Phenolic Acridine Orange Decolorizer contains concentrated hydrochloric acid (CAS 7647-01-0), 95% ethyl alcohol (CAS 64-17-5), demineralized water (CAS 7732-18-5) and methylene blue (CAS 61-73-4)
• Not all products are available for sale in all territories
• Please inquire
• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.