Thermo Scientific™ Sulfuric Acid 1% Decolorizer

Description

• Use Thermo Scientific™ Remel™ Sulfuric Acid 1% Decolorizer in the acid-fast staining procedure to detect Cryptosporidium in clinical specimens
• Cryptosporidium is an intestinal protozoan parasite that causes diarrhea in animals as well as humans
• This strain causes infection in immunocompromised hosts, especially those with AIDS (Acquired Immune Deficiency Syndrome) 1
• A modified acid-fast stain procedure was described by Ma and Soave because of its simplicity and specificity to detect Cryptosporidium 2
• Recent modification of the Kinyoun carbolfuchsin stain is recommended by Garcia for detection of Cryptosporidium oocysts in clinical specimens 3
• Cryptosporidium oocysts are spherical, 4-6 μm in diameter, and stain pink to red to purple with Kinyoun carbolfuchsin stain
• Most will contain several dark staining granules
• Yeast cells which are most likely to be confused with Cryptosporidium stain blue or green based on the counterstain used
• Sulfuric Acid 1% decolorizes the primary stain 4
• Cryptosporidium is more difficult to detect in formed stool specimens
• Stain time is increased that allows the oocysts to stand out from the background material
• The hot acid-fast stain maximizes detection and identification of Cryptosporidium in formed stools 3
• Easy-to-use - Examine smear in the slides mounted with mounting medium and a coverslip by bright field microscopy (40 X objective) and oil immersion is used (100 X objective) to observe the internal morphology
• Easy-to-interpret - In case of positive test oocysts stain pink to red to purple, are 4-6 μm in diameter and round or oval in shape
• One to four sporozoites maybe visible within the oocyst
• In case of negative test the yeast cells and background material stain blue or green in color based on the counterstain used As per procedure recommended by CLSI 4 This reagent contains 1% of sulfuric acid (CAS 7664-93-9) in demineralized water (CAS 7732-18-5) Not all products are available for sale in all territories
• Please inquire
• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.