BioTracker 625 Red GSH Dye, MilliporeSigma™

Description

• Glutathione (GSH) is an important antioxidant preventing damage to important cellular components caused by reactive oxygen species (ROS) including free radicals, peroxides, lipid peroxides, and heavy metals
• Alterations in glutathione homeostasis are associated with a variety of diseases; therefore, real-time live-cell imaging and quantification of GSH dynamics are important for understanding pathophysiological processes
• The BioTracker 625 Red GSH Dye is probe for glutathione (GSH) in living cells
• The dye is formed with an orange fluorescent dye (T) and a deep-red fluorescent dye (S)
• At a low GSH concentration, green light excitation of T, leads to an energy transfer to S and a subsequent emission of deep-red fluorescent light
• At a high GSH concentration, the energy transfer does not occur, so the probe emits the orange fluorescent light from the emission of T
• The dye produces a quick and reversible response to the GSH concentration changes, relatively slow photobleaching and low cytotoxicity
• Absorbance: 520 nm Emission: 625 nm and 582 nm