4918878
Invitrogen™ MAXIscript™ SP6 Kit with Manual
Fast synthesis of RNA probes for use in ribonuclease protection assays, in situ hybridization, and blot hybridizations
Manufacturer: Fischer Scientific
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Content And Storage
SP6 Enzyme Mix, 10X Transcription Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, TURBO DNase, Gel Loading Buffer II, and pTRI-b-actin are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
Format
Kit
For Use With (Application)
In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
Product Type
SP6 In Vitro Transcription kit
Yield
2-6 μg
Final Product Type
Synthetic mRNA, mRNA
Sample Type
mRNA, Synthetic mRNA
Product Line
Ambion™, MAXIscript™
Promoter
SP6
Starting Material
PCR Products, Linearized DNA plasmid
Related Products
Description
- Ambion MAXIscript SP6 In Vitro Transcription Kit synthesizes RNA probes in just 10 minutes for use in ribonuclease protection assays, in situ hybridization, and blot hybridizations
- Each kit contains sufficient reagents for 30 reactions
- High yields of high specific activity, full-length RNA probes in just 10 minutes (RNA Yield: 2-6μg) Ideal probe synthesis kit for nuclease protection assays, Northern and Southern blots, and in situ hybridization Includes TURBO DNase Contain quality-tested DNase/RNase-free reagents Synthesizes RNA probes with specific activities reaching 1 x 10 9 cpm/μg in just 10 minutes Efficient at synthesizing full-length probes, even at limiting nucleotide concentrations, making them perfect companion to ribonuclease protection assays For synthesis of nonisotopically labeled probes Labeling can be accomplished post-transcriptionally by using Ambion's BrightStar™ Psoralen-Biotin Labeling Kit, or during transcription by incorporating modified nucleotides into reaction For synthesis of modest amounts of unlabeled RNA (2–6μg/20μL reaction) of up to 2kb
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Content And Storage: SP6 Enzyme Mix, 10X Transcription Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, TURBO DNase, Gel Loading Buffer II, and pTRI-b-actin are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
Format: Kit
For Use With (Application): In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
Product Type: SP6 In Vitro Transcription kit
Yield: 2-6 μg
Final Product Type: Synthetic mRNA, mRNA
Sample Type: mRNA, Synthetic mRNA
Product Line: Ambion™, MAXIscript™
Promoter: SP6
Starting Material: PCR Products, Linearized DNA plasmid
Content And Storage:
SP6 Enzyme Mix, 10X Transcription Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, TURBO DNase, Gel Loading Buffer II, and pTRI-b-actin are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
Format:
Kit
For Use With (Application):
In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
Product Type:
SP6 In Vitro Transcription kit
Yield:
2-6 μg
Final Product Type:
Synthetic mRNA, mRNA
Sample Type:
mRNA, Synthetic mRNA
Product Line:
Ambion™, MAXIscript™
Promoter:
SP6
Starting Material:
PCR Products, Linearized DNA plasmid
Content And Storage: SP6 Enzyme Mix, T7 Enzyme Mix,10X Transcription Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, TURBO DNase, Gel Loading Buffer II, and pTRI-b-actin are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
Format: Kit
For Use With (Application): In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
Product Type: SP6/T7 In Vitro Transcription kit
Yield: 2-6 μg
Final Product Type: Synthetic mRNA, mRNA
Sample Type: mRNA, Synthetic mRNA
Product Line: Ambion™, MAXIscript™
Promoter: T7, SP6
Starting Material: PCR Products, Linearized DNA plasmid
Content And Storage:
SP6 Enzyme Mix, T7 Enzyme Mix,10X Transcription Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, TURBO DNase, Gel Loading Buffer II, and pTRI-b-actin are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
Format:
Kit
For Use With (Application):
In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
Product Type:
SP6/T7 In Vitro Transcription kit
Yield:
2-6 μg
Final Product Type:
Synthetic mRNA, mRNA
Sample Type:
mRNA, Synthetic mRNA
Product Line:
Ambion™, MAXIscript™
Promoter:
T7, SP6
Starting Material:
PCR Products, Linearized DNA plasmid
Content And Storage: T7 Enzyme Mix, 10X Transcription Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, TURBO DNase, Gel Loading Buffer II, and pTRI-b-actin are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
Format: Kit
For Use With (Application): In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
Product Type: T7 In Vitro Transcription kit
Yield: 2-6 μg
Final Product Type: Synthetic mRNA, mRNA
Sample Type: mRNA, Synthetic mRNA
Product Line: Ambion™, MAXIscript™
Promoter: T7
Starting Material: PCR Products, Linearized DNA plasmid
Content And Storage:
T7 Enzyme Mix, 10X Transcription Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, TURBO DNase, Gel Loading Buffer II, and pTRI-b-actin are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
Format:
Kit
For Use With (Application):
In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
Product Type:
T7 In Vitro Transcription kit
Yield:
2-6 μg
Final Product Type:
Synthetic mRNA, mRNA
Sample Type:
mRNA, Synthetic mRNA
Product Line:
Ambion™, MAXIscript™
Promoter:
T7
Starting Material:
PCR Products, Linearized DNA plasmid
Content And Storage: T7 Enzyme Mix, T3 Enzyme Mix,10X Transcription Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, TURBO DNase, Gel Loading Buffer II, and pTRI-b-actin are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
Format: Kit
For Use With (Application): In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
Product Type: T7/T3 In Vitro Transcription kit
Yield: 2-6 μg
Final Product Type: Synthetic mRNA, mRNA
Sample Type: mRNA, Synthetic mRNA
Product Line: Ambion™, MAXIscript™
Promoter: T3, T7
Starting Material: PCR Products, Linearized DNA plasmid
Content And Storage:
T7 Enzyme Mix, T3 Enzyme Mix,10X Transcription Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, TURBO DNase, Gel Loading Buffer II, and pTRI-b-actin are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
Format:
Kit
For Use With (Application):
In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
Product Type:
T7/T3 In Vitro Transcription kit
Yield:
2-6 μg
Final Product Type:
Synthetic mRNA, mRNA
Sample Type:
mRNA, Synthetic mRNA
Product Line:
Ambion™, MAXIscript™
Promoter:
T3, T7
Starting Material:
PCR Products, Linearized DNA plasmid