4918886

Invitrogen™ MAXIscript™ T7/T3 Transcription Kit

Fast synthesis of RNA probes for use in ribonuclease protection assays, in situ hybridization, and blot hybridizations

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Content And Storage

T7 Enzyme Mix, T3 Enzyme Mix,10X Transcription Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, TURBO DNase, Gel Loading Buffer II, and pTRI-b-actin are all stored at –20°C. Nuclease-free Water may be stored at any temperature.

Format

Kit

For Use With (Application)

In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products

Product Type

T7/T3 In Vitro Transcription kit

Shipping Condition

Dry Ice

Starting Material

PCR Products, Linearized DNA plasmid

Final Product Type

Synthetic mRNA, mRNA

Sample Type

mRNA, Synthetic mRNA

Product Line

Ambion™, MAXIscript™

Promoter

T3, T7

Yield

2-6 μg

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Description

  • The Ambion MAXIscript T3/T7 In Vitro Transcription Kit synthesizes RNA probes in just 10 minutes for use in ribonuclease protection assays, in situ hybridization, and blot hybridizations
  • Each kit contains sufficient reagents for 15 reactions of each enzyme
  • MAXIscript Kits are very efficient at synthesizing full-length probes, even at limiting nucleotide concentrations, making them the perfect companion to ribonuclease protection assays
  • MAXIscript Kits are also an excellent choice for the synthesis of nonisotopically labeled probes
  • Labeling can be accomplished post-transcriptionally by using Ambion's BrightStar™ Psoralen-Biotin Labeling Kit, or during transcription by incorporating modified nucleotides into the reaction
  • MAXIscript Kits can also be used for the synthesis of modest amounts of unlabeled RNA (2–6μg/20μL reaction) of up to 2kb
  • High yields of high specific activity, full-length RNA probes in just 10 minutes Ideal probe synthesis kit for nuclease protection assays, Northern and Southern blots, and in situ hybridization Includes TURBO DNase™ All kits contain quality tested DNase/RNase-free reagents The MAXIscript In Vitro Transcription Kit synthesizes RNA probes with specific activities reaching 1 ×10 9 cpm/μg in just 10 minutes

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Content And Storage:
T7 Enzyme Mix, T3 Enzyme Mix,10X Transcription Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, TURBO DNase, Gel Loading Buffer II, and pTRI-b-actin are all stored at –20°C. Nuclease-free Water may be stored at any temperature.

Format:
Kit

For Use With (Application):
In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products

Product Type:
T7/T3 In Vitro Transcription kit

Shipping Condition:
Dry Ice

Starting Material:
PCR Products, Linearized DNA plasmid

Final Product Type:
Synthetic mRNA, mRNA

Sample Type:
mRNA, Synthetic mRNA

Product Line:
Ambion™, MAXIscript™

Promoter:
T3, T7

Yield:
2-6 μg

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Store at room temperature.

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iBind

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