Novex™ ApoDETECT Annexin V-FITC Kit

Description

• The ApoDETECT Annexin V-FITC Kit provides all the reagents needed to detect the apoptotic cells
• Annexin V is a Ca 2+ -dependent phospholipid binding protein
• This protein can bind to a variety of phospholipids, but it has the highest affinity for phosphatidylserine (PS)
• The ApoDETECT Annexin V-FITC Kit binds to negatively charged phospholipid surfaces in a Ca 2+ -dependent manner
• It also prevents the formation of the prothrombinase complex, thereby inhibiting formation of thrombin (anticoagulant activity)
• The ApoDETECT Annexin V-FITC Kit can be used to detect apoptotic cells by flow cytometry or immunofluorescent cytology
• Kit Attributes: Applications: Validated application for ApoDETECT Annexin V-FITC Kit is detecting apoptotic cells by flow cytometry or immunofluorescent cytology
• Format: Vial(s) Detection Method: Fluorescence microscopy Product Size: One kit In normal cells phosphatidylserine (PS) is located on the inner leaflet of the plasma membrane
• During the early stages of apoptosis, PS is translocated to the outer layer and is exposed on the external surface of the cell
• This early event in apoptosis can be detected by using a sensitive method in which to detect PS exposure
• Translocation of PS to the external surface of the plasma membrane is not a unique property of apoptotic cells, as this phenomenon also occurs during cell necrosis
• However, during apoptosis, the cell membrane remains intact; whereas, during necrosis, the cell becomes leaky and loses its integrity
• Therefore, it is necessary to assess membrane integrity together with PS translocation
• Annexin V is a Ca 2+ -dependent phospholipid binding protein
• This protein can bind to a variety of phospholipids, but it has the highest affinity for phosphatidylserine (PS)
• Based on its affinity for PS, Annexin V can be utilized as a sensitive probe for cell surface exposure of PS
• To use the Annexin V protein as a probe for apoptotic cells, the protein has been labeled with fluorescein isothiocyanate (FITC)
• In this form, the protein can be used directly for quantification of apoptotic cells
• The measurement of Annexin V binding when performed simultaneously with a dye exclusion test (such as propidium iodide) can be used to effectively discriminate between apoptotic and necrotic cells.