Invitrogen™ NA-XTD™ Influenza Neuraminidase Assay Kit

Description

• Description The NA-XTD kit includes detection reagents and microplates, eliminating the need for luminometers equipped with a reagent injector, and improving ease-of-use
• This kit also includes complete assay protocols for quantitating sensitivity to neuraminidase inhibitors in various influenza virus isolates including: human influenza types A and B, A/H1N1 pandemic, avian, equine and porcine viruses
• Superior performance - improved, long-lived light emission kinetics and read-time flexibility High sensitivity - high assay signal-to-noise, detection of low-virus concentrations, and wide assay dynamic range Simple instrumentation requirement - no reagent injectors or luminometer needed Multiple applications - neuraminidase inhibitor IC50 assays and cell-based virus quantitation Long-Lived Light Emission Kinetics and Read-Time Flexibility The NA-XTD assay yields much longer-lived light emission kinetics than does the NA-Star assay, eliminating the need for luminometer instrumentation with reagent injectors and enabling read-time flexibility and batch-mode processing of assay plates
• NA-XTD assay signal half-life is approximately 2 hours, longer than the 5-10 minute half-life of the NA-Star assay signal
• IC-50 values determined from data collected immediately or up to 3 hours following addition of NA-XTD Accelerator solution are identical
• High Sensitivity The NA-XTD chemiluminescent assay provides higher detection sensitivity (better low-end detection limit), higher assay signal-to-noise ratio, and wider assay dynamic range than fluorescent assays with the MUNANA substrate
• The NA-XTD assay also typically demonstrates slightly higher signal-to-noise than the NA-Star assay
• The NA-XTD assay provides 2 to 50-fold higher sensitivity by signal-to-noise ratio than MUNANA-based fluorescence assays, depending on the virus isolate
• The NA-XTD assay provides a dynamic range of detection of 3 to 4 orders of magnitude of neuraminidase enzyme concentration, compared to 2 to 3 orders of magnitude range with fluorescent MUNANA assays
• The wide chemiluminescent neuraminidase assay range enables determination of IC-50 values over a wide range of virus concentrations, eliminating the need to titer virus prior to performing IC50 determination assays
• Simple Instrumentation Requirement Optimally, a small sample of culture media is removed and assayed directly with NA-XTD reagents, permitting multiple samples to be assayed over time
• Specifications Detection Method: Chemiluminescent For Use With Equipment: Luminometer (Microplate) Form: Liquid Format: 96-well plate High Throughput Compatibility: High Throughput-Compatible Readout: End Point Substrate: NA-XTD Substrate Properties: Chemical Substrate Substrate Type: Neuraminidase Substrate Target Enzyme: Neuraminidase, Sialidase