C10289

Invitrogen™ Click-iT™ AHA Alexa Fluor™ 488 Protein Synthesis HCS Assay

Manufacturer: Invitrogen™

Select a Size

Pack Size SKU Availability Price
Each of 1 C10289-Each-of-1 In Stock ₹ 67,763.52

C10289 - Each of 1

₹ 67,763.52

In Stock

Quantity

1

Base Price: ₹ 67,763.52

GST (18%): ₹ 12,197.434

Total Price: ₹ 79,960.954

Color

Blue, Green

Detection Method

Fluorescence

For Use With (Equipment)

Fluorescence Microscope, High Content Analysis Instrument

Dye Type

Alexa Fluor™ 488, Hoechst 33258

Format

96-well plate

Product Line

Alexa Fluor™, Click-iT™, Molecular Probes™

Shipping Condition

Room Temperature

Content And Storage

The kit contains sufficient reagents for performing 2 plates in a 96-well plate format.Store at ≤-20°C, desiccate and protect from light.

For Use With (Application)

High-content Screening (HCS)

Product Type

HCS Assay

Emission

UV, Visible

Green Features

Less hazardous

Quantity

2 plates

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Description

  • The Click-iT AHA Alexa Fluor 488 Protein Synthesis HCS Assay provides a fast, sensitive, non-toxic and non-radioactive method for the detection of nascent protein synthesis utilizing fluorescence microscopy and high-content imaging
  • Click-iT AHA is an amino acid analog of methionine containing an azido moiety
  • Similar to 35S-methionine, Click-iT AHA is fed to cultured cells and incorporated into proteins during active protein synthesis
  • Detection of the incorporated amine acid utilizes a chemoselectiove ligation or 'click' reaction between an azide and an alkyne, where the azido modified protein is detected with the green-fluorescent Alexa Fluor 488 alkyne
  • The Click-iT AHA Alexa Fluor 488 Protein Synthesis HCS Assay has been successfully tested in A549 and U-2 OS cells with a variety of reagents that inhibit protein synthesis including cycloheximide, anisomycin and puromycin in both dose response and Min/Max format
  • Less hazardous Cell Analysis, Cell Metabolism, Cell Viability and Cytotoxicity, Cell Viability, Proliferation and Function Order Info Shipping Condition: Room temperature

Compare Similar Items

Show Difference

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Invitrogen™

C10289

--


Color:
Blue, Green

Detection Method:
Fluorescence

For Use With (Equipment):
Fluorescence Microscope, High Content Analysis Instrument

Dye Type:
Alexa Fluor™ 488, Hoechst 33258

Format:
96-well plate

Product Line:
Alexa Fluor™, Click-iT™, Molecular Probes™

Shipping Condition:
Room Temperature

Content And Storage:
The kit contains sufficient reagents for performing 2 plates in a 96-well plate format.Store at ≤-20°C, desiccate and protect from light.

For Use With (Application):
High-content Screening (HCS)

Product Type:
HCS Assay

Emission:
UV, Visible

Green Features:
Less hazardous

Quantity:
2 plates

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Fischer Scientific

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Color:
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Detection Method:
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For Use With (Equipment):
Countess Automated Cell Counter

Dye Type:
__

Format:
__

Product Line:
Countess

Shipping Condition:
__

Content And Storage:
• Countess™ Cell Counting Chamber Slides, 10 x 50 slides• Trypan blue stain, 20 x 1 mL vialsEach slide contains two cell counting chambers.For use at room temperature under normal laboratory conditions.

For Use With (Application):
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Product Type:
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Emission:
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Green Features:
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Quantity:
500 Slides

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Color:
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Detection Method:
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For Use With (Equipment):
Countess Automated Cell Counter

Dye Type:
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Format:
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Product Line:
Countess

Shipping Condition:
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Content And Storage:
• Countess™ Cell Counting Chamber Slides, 25 x 50 slides• Trypan blue stain, 100 mL bottleEach slide contains two cell counting chambers.For use at room temperature under normal laboratory conditions.

For Use With (Application):
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Emission:
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Green Features:
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1250 Slides

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Fischer Scientific

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Color:
__

Detection Method:
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For Use With (Equipment):
Countess Automated Cell Counter

Dye Type:
__

Format:
__

Product Line:
Countess

Shipping Condition:
__

Content And Storage:
• Countess™ Cell Counting Chamber Slides, 50 x 50 slides• Trypan blue stain, 100 mL bottleEach slide contains two cell counting chambers.For use at room temperature under normal laboratory conditions.

For Use With (Application):
__

Product Type:
__

Emission:
__

Green Features:
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Quantity:
2500 Slides