C10337

Invitrogen™ Click-iT™ EdU Cell Proliferation Kit for Imaging, Alexa Fluor™ 488 dye

Manufacturer: Invitrogen™

Select a Size

Pack Size SKU Availability Price
Each of 1 C10337-Each-of-1 In Stock ₹ 69,988.08

C10337 - Each of 1

₹ 69,988.08

In Stock

Quantity

1

Base Price: ₹ 69,988.08

GST (18%): ₹ 12,597.854

Total Price: ₹ 82,585.934

Color

Green

Detection Method

Fluorescence

For Use With (Equipment)

Confocal Microscope, Fluorescence Microscope

Dye Type

Alexa Fluor™ 488

Format

Slide(s)

Product Line

Alexa Fluor™, Click-iT™, Molecular Probes™

Shipping Condition

Room Temperature

Content And Storage

Each kit contains enough material for 50 to 250 reactions, depending on reaction volume (typically between 0.1 and 0.5 ml). Store at 2–6°C, dessicate and protect from light.

For Use With (Application)

Proliferation Assay

Product Type

Cell Proliferation Kit

Emission

Visible

Green Features

Less hazardous

Quantity

1 kit

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Description

  • The Click-iT EdU Alexa Fluor 488 Imaging Kit is a superior alternative to traditional proliferation assays that is optimized for fluorescence microscopy applications
  • In this assay the modified thymidine analogue EdU is efficiently incorporated into newly synthesized DNA and fluorescently labeled with a bright, photostable Alexa Fluor dye in a fast, highly specific click reaction
  • This fluorescent labeling of proliferating cells is accurate and compatible with antibody methods due to the mild click protocol
  • The most accurate proliferation-detection methods are based on the incorporation and measurement of nucleoside analogues in newly synthesized DNA, with bromodeoxyuridine (BrdU) a commonly used analogue
  • BrdU-labeled DNA is quantitated using anti-BrdU antibodies following DNA denaturation by harsh methods (HCl, heat, or enzymes) to expose the BrdU molecules
  • This step is time consuming and difficult to perform consistently
  • The harsh treatment can also adversely effect sample integrity and quality, which makes co-staining with other antibodies challenging
  • Simple: works the first time, every time, in less time Efficient: no denaturation steps or harsh treatment required Content-rich results: better preservation of cell morphology, antigen structure, and DNA integrity Consistent: not dependent on variable antibody lots for detection Less hazardous The Click-iT assay can be used on cells in culture or in vivo following EdU administration by feeding or injection methods
  • The Click-iT assay can be used with BrdU in dual pulse experiments by using the anti-BrdU (clone MoBu-1) antibody, which does not cross react with EdU
  • The Click-iT technology is compatible with immunohistochemical, immunocytochemical, and fluorescent dyes that are fixation tolerant or designed for fixed cell labeling
  • Cell Analysis, Cell Cycle, Cell Proliferation, Cell Viability, Proliferation and Function, Cellular Imaging, DNA Replication and Repair, Immunocytochemistry (ICC), Immunofluorescence (IF) Order Info Shipping Condition: Room temperature

Compare Similar Items

Show Difference

Img

Invitrogen™

C10337

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Color:
Green

Detection Method:
Fluorescence

For Use With (Equipment):
Confocal Microscope, Fluorescence Microscope

Dye Type:
Alexa Fluor™ 488

Format:
Slide(s)

Product Line:
Alexa Fluor™, Click-iT™, Molecular Probes™

Shipping Condition:
Room Temperature

Content And Storage:
Each kit contains enough material for 50 to 250 reactions, depending on reaction volume (typically between 0.1 and 0.5 ml). Store at 2–6°C, dessicate and protect from light.

For Use With (Application):
Proliferation Assay

Product Type:
Cell Proliferation Kit

Emission:
Visible

Green Features:
Less hazardous

Quantity:
1 kit

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Invitrogen™

C10338

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Color:
Orange

Detection Method:
Fluorescence

For Use With (Equipment):
Fluorescence Microscope

Dye Type:
Alexa Fluor™ 555

Format:
Slide(s)

Product Line:
Alexa Fluor™, Click-iT™

Shipping Condition:
Room Temperature

Content And Storage:
Each kit contains enough material for 50 to 250 reactions, depending on reaction volume (typically between 0.1 and 0.5 ml). Store at 2–6°C, dessicate and protect from light.

For Use With (Application):
Proliferation Assay

Product Type:
Cell Proliferation Kit

Emission:
Visible

Green Features:
Less hazardous

Quantity:
1 kit

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Invitrogen™

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Color:
Red

Detection Method:
Fluorescence

For Use With (Equipment):
Confocal Microscope, Fluorescence Microscope

Dye Type:
Alexa Fluor™ 594

Format:
Slide(s)

Product Line:
Alexa Fluor™, Click-iT™, Molecular Probes™

Shipping Condition:
Room Temperature

Content And Storage:
Each kit contains enough material for 50 to 250 reactions, depending on reaction volume (typically between 0.1 and 0.5 ml). Store at 2–6°C, dessicate and protect from light.

For Use With (Application):
Proliferation Assay

Product Type:
Cell Proliferation Kit

Emission:
Visible

Green Features:
Less hazardous

Quantity:
1 kit

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Invitrogen™

C10340

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Color:
Far-Red

Detection Method:
Fluorescence

For Use With (Equipment):
Confocal Microscope, Fluorescence Microscope

Dye Type:
Alexa Fluor™ 647

Format:
Slide(s)

Product Line:
Alexa Fluor™, Click-iT™, Molecular Probes™

Shipping Condition:
Room Temperature

Content And Storage:
The kit contains sufficient material for 50 18x18 coverslips. Store at 2°C to 6°C, dessicate and protect from light.

For Use With (Application):
Proliferation Assay

Product Type:
Cell Proliferation Kit

Emission:
near IR

Green Features:
Less hazardous

Quantity:
1 kit