50-112-1508
Molecular Probes™ Violet Ratiometric Membrane Asymmetry Probe/Dead Cell Apoptosis Kit, for flow cytometry
Manufacturer: Molecular Probes™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-112-1508-Each-of-1 | In Stock | ₹ 42,009.96 |
50-112-1508 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 42,009.96
GST (18%): ₹ 7,561.793
Total Price: ₹ 49,571.753
Quantity
1 kit
Product Type
Dead Cell Apoptosis Kit
Flow Cytometer Laser Lines
405, 488
No. of Reactions
100
Detection Method
Fluorescence
For Use With (Equipment)
Flow Cytometer
Label Type
Other Label(s) or Dye(s)
Format
Tube
Content And Storage
Contains 1 vial of F2N12S (100 μL), 1 vial of SYTOX™ AADvanced™ dead cell stain, and 1 vial of DMSO (200 μL).Store at -20°C, dessicate and protect from light.
Excitation/Emission
SYTOX™ AADvanced™: 546/647F2N12S: 405/530
Shipping Condition
Dry Ice
For Use With (Application)
Flow Cytometry
Label or Dye
F2N12S, SYTOX AADvanced Dead Cell Stain
Related Products
Description
- The Violet Ratiometric Membrane Asymmetry Probe/Dead Cell Apoptosis Kit provides a simple and fast method for the detection of apoptosis with dead cell discrimination by flow cytometry
- The Violet Ratiometric Membrane Asymmetry Probe, 4'-N,N-diethylamino-6-(N,N,N-dodecyl-methylamino-sulfopropyl)-methyl-3-hydroxyflavone (F2N12S), is a novel violet excitable dye for the detection of membrane asymmetry changes during apoptosis
- The dye exhibits an excited-state intramolecular proton transfer (ESIPT) reaction resulting in a dual fluorescence with two emission bands corresponding to 530 nm and 585 nm, producing a two-color ratiometric response to variations in surface charge
- Ratiometric probes have several advantages over traditional fluorochrome labeled reagents
- The ratiometric probe is a self-calibrating absolute parameter of apoptotic transformation, which is independent of probe concentration, cell size, and instrument variation, such as fluctuations of laser intensity or sensitivity of the detectors
- Given that apoptosis modifies the surface charge of the outer leaflet of the plasma membrane the violet membrane asymmetry probe F2N12S can monitor changes in membrane asymmetry that occur during apoptosis through a change in the relative intensity of the two emission bands of the dye
- The F2N12S probe is combined with SYTOX(R) AADvanced dead cell stain, which is capable of passing through the cell membrane only in late apoptotic or necrotic cells allowing discrimination form early apoptotic cells
- Samples can be analyzed after a 5 minute incubation at room temperature and does not require special buffers or wash steps
- This kit can be paired with other reagents such as MitoProbe™ DiIC1(5) or annexin V for multiparametric analysis of apoptosis and viability
- This reagent kit allow researchers to maximaize the utility of their instruments by utilizing the violet laser Each kit contains sufficient reagents for ∼100 flow cytometry tests
- View a selection guide for all apoptosis assays for flow cytometry .
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Quantity: 1 kit
Product Type: Dead Cell Apoptosis Kit
Flow Cytometer Laser Lines: 405, 488
No. of Reactions: 100
Detection Method: Fluorescence
For Use With (Equipment): Flow Cytometer
Label Type: Other Label(s) or Dye(s)
Format: Tube
Content And Storage: Contains 1 vial of F2N12S (100 μL), 1 vial of SYTOX™ AADvanced™ dead cell stain, and 1 vial of DMSO (200 μL).Store at -20°C, dessicate and protect from light.
Excitation/Emission: SYTOX™ AADvanced™: 546/647F2N12S: 405/530
Shipping Condition: Dry Ice
For Use With (Application): Flow Cytometry
Label or Dye: F2N12S, SYTOX AADvanced Dead Cell Stain
Quantity:
1 kit
Product Type:
Dead Cell Apoptosis Kit
Flow Cytometer Laser Lines:
405, 488
No. of Reactions:
100
Detection Method:
Fluorescence
For Use With (Equipment):
Flow Cytometer
Label Type:
Other Label(s) or Dye(s)
Format:
Tube
Content And Storage:
Contains 1 vial of F2N12S (100 μL), 1 vial of SYTOX™ AADvanced™ dead cell stain, and 1 vial of DMSO (200 μL).Store at -20°C, dessicate and protect from light.
Excitation/Emission:
SYTOX™ AADvanced™: 546/647F2N12S: 405/530
Shipping Condition:
Dry Ice
For Use With (Application):
Flow Cytometry
Label or Dye:
F2N12S, SYTOX AADvanced Dead Cell Stain
Quantity: 20 x 50 μg
Product Type: Bacterial Stain
Flow Cytometer Laser Lines: __
No. of Reactions: __
Detection Method: Fluorescence
For Use With (Equipment): Fluorescence Microscope, Flow Cytometer
Label Type: __
Format: Tube(s), Slide(s)
Content And Storage: Contains 20 vials of BacLight™ red bacterial stain (50 ug per vial).Store in freezer (-5 to -30°C) and protect from light.
Excitation/Emission: __
Shipping Condition: Room Temperature
For Use With (Application): __
Label or Dye: __
Quantity:
20 x 50 μg
Product Type:
Bacterial Stain
Flow Cytometer Laser Lines:
__
No. of Reactions:
__
Detection Method:
Fluorescence
For Use With (Equipment):
Fluorescence Microscope, Flow Cytometer
Label Type:
__
Format:
Tube(s), Slide(s)
Content And Storage:
Contains 20 vials of BacLight™ red bacterial stain (50 ug per vial).Store in freezer (-5 to -30°C) and protect from light.
Excitation/Emission:
__
Shipping Condition:
Room Temperature
For Use With (Application):
__
Label or Dye:
__
Quantity: 100 assays
Product Type: __
Flow Cytometer Laser Lines: __
No. of Reactions: __
Detection Method: __
For Use With (Equipment): __
Label Type: __
Format: Tube
Content And Storage: __
Excitation/Emission: CellROX orange: 545/565, SYTOX red: 640/658
Shipping Condition: __
For Use With (Application): __
Label or Dye: __
Quantity:
100 assays
Product Type:
__
Flow Cytometer Laser Lines:
__
No. of Reactions:
__
Detection Method:
__
For Use With (Equipment):
__
Label Type:
__
Format:
Tube
Content And Storage:
__
Excitation/Emission:
CellROX orange: 545/565, SYTOX red: 640/658
Shipping Condition:
__
For Use With (Application):
__
Label or Dye:
__
Quantity: __
Product Type: __
Flow Cytometer Laser Lines: __
No. of Reactions: __
Detection Method: __
For Use With (Equipment): __
Label Type: __
Format: __
Content And Storage: Contains EdU (5-ethynyl-2' -deoxyuridine), AlexaFluor 594 picolyl azide, anhydrous dimethylsulfoxide (DMSO), Click-iT fixative, Click-iT saponin-based permeabilization and wash buffer, Copper Protectant, and Click-iT EdU buffer additive.Store at 2–8°C. Dessicate and protect from light.
Excitation/Emission: 532 or 561⁄615
Shipping Condition: Room Temperature
For Use With (Application): __
Label or Dye: __
Quantity:
__
Product Type:
__
Flow Cytometer Laser Lines:
__
No. of Reactions:
__
Detection Method:
__
For Use With (Equipment):
__
Label Type:
__
Format:
__
Content And Storage:
Contains EdU (5-ethynyl-2' -deoxyuridine), AlexaFluor 594 picolyl azide, anhydrous dimethylsulfoxide (DMSO), Click-iT fixative, Click-iT saponin-based permeabilization and wash buffer, Copper Protectant, and Click-iT EdU buffer additive.Store at 2–8°C. Dessicate and protect from light.
Excitation/Emission:
532 or 561⁄615
Shipping Condition:
Room Temperature
For Use With (Application):
__
Label or Dye:
__