Invitrogen™ eBioscience™ Phalloidin eFluor™ 520

Description

• Phalloidin is a peptide toxin isolated from the Amanita phalloides mushroom
• Phalloidin selectively binds F-actin and functions to stablilize actin polymers by inhibiting the dissociation of actin monomers from the filament ends
• Because F-actin polymers compose the cytoskeleton of almost all eukaryotic cells, phalloidin conjugates can be useful probes for visualizing the structure of live or fixed cells using microscopy
• Phalloidin eFluor 520 can be used to label cells of multiple species
• Its exitation and emission is similar to Alexa Fluor 488 and FITC
• Phalloidin eFluor 520 is supplied as a frozen liquid
• To prepare the 1000X stock solution, thaw the vial, add 30μL high-quality anhydrous DMSO, mix and briefly spin down
• The resulting solution (phalloidin DMSO stock) should be aliquoted, protected from light and moisture, and stored at -20°C
• To prepare a 1X working solution of phalloidin conjugate, add 1μL of 1000X phalloidin DMSO stock solution to 1mL of PBS containing 1% BSA.