Invitrogen™ eBioscience™ Phalloidin eFluor™ 660

Description

• Phalloidin is a peptide toxin isolated from the Amanita phalloides mushroom
• Phalloidin selectively binds F-actin and functions to stablilize actin polymers by inhibiting the dissociation of actin monomers from the filament ends
• Because F-actin polymers compose the cytoskeleton of almost all eukaryotic cells, phalloidin conjugates can be useful probes for visualizing the structure of live or fixed cells using microscopy
• Phalloidin eFluor 660 can be used to label cells of multiple species
• Phalloidin eFluor 660 is supplied as a lyophilized solid and should be prepared by reconstitution with 30μL high-quality anhydrous DMSO to give a 1000X stock solution
• The phalloidin DMSO stock solution should be aliquoted, protected from light and moisture, and stored at -20°C
• To prepare a 1X working solution of phalloidin conjugate, add 1μL of 1000X phalloidin conjugate DMSO solution to 1mL of PBS containing 1% BSA
• Phalloidin is a peptide toxin isolated from the Amanita phalloides mushroom
• Phalloidin selectively binds F-actin and functions to stabilize actin polymers by inhibiting the dissociation of actin monomers from the filament ends
• Because F-actin polymers compose the cytoskeleton of almost all eukaryotic cells, phalloidin conjugates can be useful probes for visualizing the structure of live or fixed cells using microscopy
• Phalloidin eFluor™ 660 is supplied as a lyophilized solid To prepare the 1000X stock solution, the material should be mixed with 30μL high-quality anhydrous DMSO The resulting phalloidin DMSO stock should be aliquoted, protected from light and moisture, and stored at −20°C To prepare a 1X working solution of phalloidin conjugate, add 1μL of 1000X phalloidin DMSO stock solution to 1mL of PBS containing 1% BSA