50-112-4447

IFN gamma Monoclonal Antibody (DB-1 (DB1)), eFluor™ 660, eBioscience™, Invitrogen™

Manufacturer: Invitrogen

Select a Size

Pack Size SKU Availability Price
Each of 1 50-112-4447-Each-of-1 In Stock ₹ 10,780.56

50-112-4447 - Each of 1

₹ 10,780.56

In Stock

Quantity

1

Base Price: ₹ 10,780.56

GST (18%): ₹ 1,940.501

Total Price: ₹ 12,721.061

Antigen

IFN gamma

Classification

Monoclonal

Concentration

0.2 mg/mL

Formulation

PBS with 0.09% sodium azide; pH 7.2

Gene Accession No.

P01581

Gene Symbols

IFNG

Purification Method

Affinity chromatography

Regulatory Status

RUO

Gene ID (Entrez)

25712

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Form

Liquid

Applications

Flow Cytometry

Clone

DB-1 (DB1)

Conjugate

eFluor 660

Gene

IFNG

Gene Alias

BoIFNG; cytokine; EGK_03901; gamma interferon; gamma interferon precursor; gamma-interferon; gamma-interferon precursor (AA -23 to 143); H-IFN-g; IFG; IFI; IFN gamma; IFN ?; Ifng; IFN-g; IFNG2; IFN-gamma; IFN-gamma precursor; IFN-y; IFN?; Immune interferon; interferon gamma; interferon gamma precursor; interferon gamma type 2; Interferon y; Interferon ?; interferon, gamma; interferon-gama; interferon-gamma; Interferon-gamma level; interferon-gamma precursor; Interferon?; M-IFN-g; R-IFN-g

Host Species

Mouse

Quantity

25 μg

Primary or Secondary

Primary

Target Species

Rat

Product Type

Antibody

Isotype

IgG1 κ

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Description

  • Description: The DB-1 monoclonal antibody reacts with rat interferon gamma, a 15-20 kDa cytokine secreted by Th1 cells
  • Applications Reported: This DB-1 (DB1) antibody has been reported for use in intracellular staining followed by flow cytometric analysis
  • Applications Tested: This DB-1 (DB1) antibody has been tested by intracellular staining and flow cytometric analysis of stimulated rat splenocytes
  • This can be used at less than or equal to 0.5 μg per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • eFluor™ 660 is a replacement for Alexa Fluor™ 647
  • eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm)
  • Please make sure that your instrument is capable of detecting this fluorochome
  • Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser
  • Filtration: 0.2 μm post-manufacturing filtered
  • IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines
  • IFN gamma is a dimeric protein consisting of two 146 amino acid subunits
  • IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa
  • On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation
  • The biological activity of the IFN gamma homodimer is highly species specific
  • Human IFN gamma does not show cross-reactivity with mouse
  • IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes
  • IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines
  • Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway
  • IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma
  • IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN
  • IFN gamma gene mutations are associated with aplastic anemia.

Compare Similar Items

Show Difference

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Invitrogen

50-112-4447

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Antigen:
IFN gamma

Classification:
Monoclonal

Concentration:
0.2 mg/mL

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Accession No.:
P01581

Gene Symbols:
IFNG

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
25712

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

Applications:
Flow Cytometry

Clone:
DB-1 (DB1)

Conjugate:
eFluor 660

Gene:
IFNG

Gene Alias:
BoIFNG; cytokine; EGK_03901; gamma interferon; gamma interferon precursor; gamma-interferon; gamma-interferon precursor (AA -23 to 143); H-IFN-g; IFG; IFI; IFN gamma; IFN ?; Ifng; IFN-g; IFNG2; IFN-gamma; IFN-gamma precursor; IFN-y; IFN?; Immune interferon; interferon gamma; interferon gamma precursor; interferon gamma type 2; Interferon y; Interferon ?; interferon, gamma; interferon-gama; interferon-gamma; Interferon-gamma level; interferon-gamma precursor; Interferon?; M-IFN-g; R-IFN-g

Host Species:
Mouse

Quantity:
25 μg

Primary or Secondary:
Primary

Target Species:
Rat

Product Type:
Antibody

Isotype:
IgG1 κ

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Invitrogen

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Antigen:
IFN gamma

Classification:
Monoclonal

Concentration:
0.2 mg/mL

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Accession No.:
P01580

Gene Symbols:
IFNG

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
15978

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

Applications:
Flow Cytometry

Clone:
XMG1.2

Conjugate:
eFluor 660

Gene:
IFNG

Gene Alias:
BoIFNG; cytokine; EGK_03901; gamma interferon; gamma interferon precursor; gamma-interferon; gamma-interferon precursor (AA -23 to 143); H-IFN-g; IFG; IFI; IFN gamma; IFN ?; Ifng; IFN-g; IFNG2; IFN-gamma; IFN-gamma precursor; IFN-y; IFN?; Immune interferon; interferon gamma; interferon gamma precursor; interferon gamma type 2; Interferon y; Interferon ?; interferon, gamma; interferon-gama; interferon-gamma; Interferon-gamma level; interferon-gamma precursor; Interferon?; M-IFN-g; R-IFN-g

Host Species:
Rat

Quantity:
100 μg

Primary or Secondary:
Primary

Target Species:
Mouse

Product Type:
Antibody

Isotype:
IgG1 κ

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Antigen:
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Classification:
Monoclonal

Concentration:
0.2 mg/mL

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Accession No.:
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Gene Symbols:
IL12A

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
16159

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

Applications:
Flow Cytometry

Clone:
4D10p35

Conjugate:
eFluor 660

Gene:
IL12A

Gene Alias:
CLMF; CLMF p35; cytotoxic lymphocyte maturation factor 1, p35; cytotoxic lymphocyte maturation factor 35 kDa subunit; il 12; IL-12 subunit p35; IL-12, subunit p35; Il12a; IL-12A; IL12p35; IL-12p35; IL35 subunit; ILN; Interleukin; Interleukin 12; interleukin 12 p35 subunit; interleukin 12, p35; interleukin 12a; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); Interleukin12; interleukin-12 alpha chain; interleukin-12 beta p35 subunit; interleukin-12 p35 subunit; interleukin-12 subunit alpha; Ll12a; NF cell stimulatory factor chain 1; NFSK; NK cell stimulatory factor chain 1; NKSF1; P35

Host Species:
Rat

Quantity:
25 μg

Primary or Secondary:
Primary

Target Species:
Mouse

Product Type:
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Classification:
Monoclonal

Concentration:
0.2 mg/mL

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Accession No.:
P15247

Gene Symbols:
Il9

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
16198

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

Applications:
Flow Cytometry

Clone:
RM9A4

Conjugate:
eFluor 660

Gene:
Il9

Gene Alias:
cytokine P40; homolog of mouse T cell and mast cell growth factor 40; HP40; IL9; IL-9; ILN; Interleukin; interleukin 9; Interleukin9; interleukin-9; P40; p40 cytokine; p40 T-cell and mast cell growth factor; T-cell growth factor P40

Host Species:
Rat

Quantity:
100 μg

Primary or Secondary:
Primary

Target Species:
Mouse

Product Type:
Antibody

Isotype:
IgG1 κ