Invitrogen™ FluxOR™ II Green Potassium Ion Channel Assay

Description

• The FluxOR II Green assay takes advantage of the permeability of potassium channels to thallium ions
• It utilizes an improved thallium-sensitive fluorogenic indicator dye that results in a larger signal window and a significant improvement in the sensitivity of the assay
• Also, the assay has been configured to be performed in either a Wash or No Wash format
• Features include: • Improved assay sensitivity and larger signal window facilitate analysis of a wider range of potassium channels, including low-expressed or weakly conducting channels • 100% increase in dynamic range compared to previous generation; >40% increase in dynamic range compared to competition • Homogenous, no-wash protocol reduces well-to-well variation and improves assay reproducibility • Optimized for high throughput screening applications • Formatted for low- to high-throughput users Similar to the previous generation assay, the FluxOR II Green Potassium Ion Channel Assay makes use of the permeability of potassium channels to thallium
• When potassium channels are opened by a stimulus, extracellular thallium flows down its concentration gradient into the cells, and channel or transporter activity is detected with a proprietary indicator dye pre-loaded into the cells that increases in cytosolic fluorescence in the presence of thallium
• In this way, the fluorescence observed using the FluxOR II assay is an indicator of any ion channel activity or transport process that allows thallium into cells
• This fluorogenic signal quantitatively reflects the activity of ion channels and transporters that are permeant to thallium, including hERG, Kir2.1, and other pharmacologically important potassium channels
• For the FluxOR II assay, an improved indicator dye was developed, resulting in higher signal windows and improved assay sensitivity
• In addition to an improved fluorescent indicator dye, the FluxOR II Green Potassium Ion Channel Assay provides a concentrated thallium solution and all necessary buffers, allowing maximum target flexibility and ease of operation in a homogenous format that has been demonstrated with cells stably expressing hERG
• The assay can be configured for Wash and No Wash formats.