Molecular Probes™ Fluo-4FF, Pentapotassium Salt, cell impermeant

Description

• Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca 2+
• Fluo-5F, fluo-5N, and fluo-4ff are analogs of fluo-4 with lower Ca 2+ -binding affinity, making them suitable for detecting intracellular calcium levels in the 1μM to 1 mM range that would saturate the response of fluo-3 and fluo-4
• Cells may be physically loaded with the cell-impermeant salt forms of these indicators using patch pipette or microinjection or our Influx™ pinocytotic cell-loading reagent
• These indicators are compatible with excitation at 488 nm by argon-ion laser sources, making them useful for confocal microscopy, flow cytometry, and microplate screening applications
• Calcium Indicator (Cell-Impermeant Salts) Specifications: Label (Ex/Em of Ca 2+ –bound form): Fluo-4FF (494/516 nm) Fluorescence intensity increase upon binding Ca 2+ : >100 fold K d for Ca 2+ in buffer: ∼9.7μM Exhibit fluorescence increase upon binding Ca 2+ with little shift in wavelength Using TPEN to Control Heavy Metal Cations In addition, BAPTA-based indicators such as these bind various heavy metal cations (e.g., Mn 2+ , Zn 2+ , Pb 2+ ) with substantially higher affinity than Ca 2+
• Perturbations to calcium measurements caused by presence of these ions can be controlled using the heavy metal-selective chelator TPEN
• More Choices for Fluorescent Calcium Indicators We offer a large selection of Molecular Probes™ calcium indicators for use in various experimental scenarios
• For more information, review Fluorescent Ca 2+ Indicators Excited with Visible Light—Section 19.3 in the Molecular Probes™ Handbook
• For UV-excitable Ca 2+ indicators, protein-based Ca 2+ indicators, conjugates of Ca 2+ indicators, and for fluorescence-based indicators of other metal ions (i.e., Mg 2+ , Zn 2+ ) review Indicators for Ca 2+ , Mg 2+ , Zn 2+ and Other Metal Ions—Chapter 19 in the Molecular Probes™ Handbook
• For Research Use Only
• Not for human or animal therapeutic or diagnostic use.