Molecular Probes™ Fluo-4, AM, cell permeant

Description

• Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca 2+
• Fluo-3 has been used to image the spatial dynamics of Ca 2+ signaling, in flow cytometry experiments involving photoactivation of caged chelators, second messengers, and neurotransmitters, and for cell-based pharmacological screening
• Fluo-4 is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels
• Cells may be loaded with the AM ester forms of these calcium indicators by adding the dissolved indicator directly to dishes containing cultured cells
• These indicators are useful for fluorescence and confocal microscopy, flow cytometry, and microplate screening applications
• Calcium Indicator (AM Ester) Specifications: • Label (Ex/Em of Ca 2+ –bound form): Fluo-4 (494/506 nm) • Fluorescence intensity increase upon binding Ca 2+ : >100 fold • K d for Ca 2+ in buffer: ∼335 nM • Exhibit fluorescence increase upon binding Ca 2+ with little shift in wavelength Using TPEN to Control Heavy Metal Cations In addition, BAPTA-based indicators such as these bind various heavy metal cations (e.g., Mn 2+ , Zn 2+ , Pb 2+ ) with substantially higher affinity than Ca 2+
• Perturbations to calcium measurements caused by presence of these ions can be controlled using the heavy metal-selective chelator TPEN (Cat
• No
• T1210)
• More Choices for Fluorescent Calcium Indicators We offer a large selection of Molecular Probes™ calcium indicators for use in various experimental scenarios
• For more information, review Fluorescent Ca 2+ Indicators Excited with Visible Light—Section 19.3 in the Molecular Probes™ Handbook
• For UV-excitable Ca 2+ indicators, protein-based Ca 2+ indicators, conjugates of Ca 2+ indicators, and for fluorescence-based indicators of other metal ions (i.e., Mg 2+ , Zn 2+ ) review Indicators for Ca 2+ , Mg 2+ , Zn 2+ and Other Metal Ions—Chapter 19 in the Molecular Probes™ Handbook
• In reduced state, the excitation and emission maxima of BODIPY™ 581/591 undecanoic acid is 581/591 nm; after oxidation, the probe shifts the excitation and emission to 488/510 nm
• We recommend dissolving in high-quality anhydrous DMSO for stock concentrations of 1 to 5 mM.