Thermo Scientific™ Rhod-3 Calcium Imaging Kit

Description

• The novel Rhod-3 Calcium Imaging Kit is designed for live-cell imaging of cytosolic calcium signaling in combination with green-fluorescent dyes or proteins.The red-fluorescent Rhod-3 AM dye supplied in the Rhod-3 Calcium Imaging Kit offers significant improvements over existing Ca 2+ -sensing dyes
• The uptake into organelles has been reduced, which translates into better cytosolic localization
• Also, Rhod-3 AM exhibits a large increase in fluorescence emission upon binding to calcium, making it more sensitive than other red-fluorescent calcium dyes
• Compared to existing red calcium dyes, Rhod-3 AM exhibits a large increase (>2.5 fold) in fluorescence upon binding Ca 2+ and very low fluorescence at rest (without Ca 2+ binding)
• Narrow emission enables simultaneous detection with GFP or other dyes
• Enhanced cytosolic localization for better detection of cytosolic transients For easy cell loading, the Rhod-3 Calcium Imaging Kit contains PowerLoad™ Concentrate
• Due to the unique nature of the PowerLoad™ solution, it can be used in the presence of complete culture media, thus reducing the negative effects of replacing media or loading in serum-free media
• A proprietary, water-soluble form of probenecid (which is commonly used to inhibit cellular transport and thus reduces the baseline signal) is also supplied with the Rhod-3 Calcium Imaging Kit
• Calcium indicators are important tools in signal transduction and cell-based pharmacological screening
• In cells and tissues with blue or green autofluorescence, long-wavelength (i.e., red-shifted) calcium indicators provide a means to bypass this overlapping autofluorescence
• Additionally, red-shifted calcium dyes are well suited for calcium imaging experiments multiplexed with green-fluorescent protein (GFP) or other green fluorescent dyes
• The Rhod-3 AM dye (included in the Rhod-3 Calcium Imaging Kit) is an improved red-shifted rhodamine-based calcium dye with an attached AM ester group
• Once inside the cell, intracellular esterases cleave the AM group, trapping the dye in the cell and rendering the dye fluorescent
• As calcium binds, the fluorescence intensity of the dye is enhanced
• The spectral properties of Rhod-3 AM (ex/em = ∼560/600 nm) and strong fluorescence upon calcium binding provide an excellent signal window and make it our brightest red calcium dye
• Additionally, Rhod-3 AM displays a more uniform cytosolic distribution and improved signal compared to existing red calcium dyes such as Rhod-2.