F1242
Molecular Probes™ Fluo-3, AM, Calcium Indicator
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | F1242-Each-of-1 | In Stock | ₹ 44,856.00 |
F1242 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 44,856.00
GST (18%): ₹ 8,074.08
Total Price: ₹ 52,930.08
Cell Permeability
Cell-Permeant
Detection Method
Fluorescence
For Use With (Equipment)
Confocal Microscope, Fluorescence Microscope, High Content Analysis Instrument, HTS Reader, Microplate Reader, Fluorescent Imager
Dye Type
Fluorescent Dye-Based
Indicator Properties
Fluorescent Dye-Based
Shipping Condition
Room Temperature
Content And Storage
Store in freezer -5°C to -30°C and protect from light.
For Use With (Application)
Calcium Assay
Product Type
Stain
Indicator
Calcium Indicator
Quantity
20 x 50μg
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Description
- Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca 2+
- Fluo-3 has been used to image the spatial dynamics of Ca 2+ signaling, in flow cytometry experiments involving photoactivation of caged chelators, second messengers, and neurotransmitters, and for cell-based pharmacological screening
- Fluo-4 is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels
- Cells may be loaded with the AM ester forms of these calcium indicators by adding the dissolved indicator directly to dishes containing cultured cells
- These indicators are useful for fluorescence and confocal microscopy, flow cytometry, and microplate screening applications
- Calcium Indicator (AM Ester) Specifications: Label (Ex/Em of Ca 2+ –bound form): Fluo-3 (506/526 nm) Fluorescence intensity increase upon binding Ca 2+ : >100 fold K d for Ca 2+ in buffer: ∼335 nM Exhibit fluorescence increase upon binding Ca 2+ with little shift in wavelength Using TPEN to Control Heavy Metal Cations In addition, BAPTA-based indicators such as these bind various heavy metal cations (e.g., Mn 2+ , Zn 2+ , Pb 2+ ) with substantially higher affinity than Ca 2+
- Perturbations to calcium measurements caused by presence of these ions can be controlled using the heavy metal-selective chelator TPEN
- More Choices for Fluorescent Calcium Indicators We offer a large selection of Molecular Probes™ calcium indicators for use in various experimental scenarios
- For more information, review Fluorescent Ca 2+ Indicators Excited with Visible Light—Section 19.3 in the Molecular Probes™ Handbook
- For UV-excitable Ca 2+ indicators, protein-based Ca 2+ indicators, conjugates of Ca 2+ indicators, and for fluorescence-based indicators of other metal ions (i.e., Mg 2+ , Zn 2+ ) review Indicators for Ca 2+ , Mg 2+ , Zn 2+ and Other Metal Ions—Chapter 19 in the Molecular Probes™ Handbook
- For Research Use Only
- Not for human or animal therapeutic or diagnostic use.
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Cell Permeability: Cell-Permeant
Detection Method: Fluorescence
For Use With (Equipment): Confocal Microscope, Fluorescence Microscope, High Content Analysis Instrument, HTS Reader, Microplate Reader, Fluorescent Imager
Dye Type: Fluorescent Dye-Based
Indicator Properties: Fluorescent Dye-Based
Shipping Condition: Room Temperature
Content And Storage: Store in freezer -5°C to -30°C and protect from light.
For Use With (Application): Calcium Assay
Product Type: Stain
Indicator: Calcium Indicator
Quantity: 20 x 50μg
Cell Permeability:
Cell-Permeant
Detection Method:
Fluorescence
For Use With (Equipment):
Confocal Microscope, Fluorescence Microscope, High Content Analysis Instrument, HTS Reader, Microplate Reader, Fluorescent Imager
Dye Type:
Fluorescent Dye-Based
Indicator Properties:
Fluorescent Dye-Based
Shipping Condition:
Room Temperature
Content And Storage:
Store in freezer -5°C to -30°C and protect from light.
For Use With (Application):
Calcium Assay
Product Type:
Stain
Indicator:
Calcium Indicator
Quantity:
20 x 50μg
Cell Permeability: __
Detection Method: __
For Use With (Equipment): __
Dye Type: __
Indicator Properties: __
Shipping Condition: Wet or Dry Ice
Content And Storage: • Phire Hot Start II DNA Polymerase, 1 x 1 mL• 5X Phire Green Reaction Buffer, 7 x 1.5 mL• DMSO, 1 x 500 μLStore at -20°C.
For Use With (Application): Cloning, Hot-start PCR
Product Type: Hot Start High-Fidelity DNA Polymerase
Indicator: __
Quantity: __
Cell Permeability:
__
Detection Method:
__
For Use With (Equipment):
__
Dye Type:
__
Indicator Properties:
__
Shipping Condition:
Wet or Dry Ice
Content And Storage:
• Phire Hot Start II DNA Polymerase, 1 x 1 mL• 5X Phire Green Reaction Buffer, 7 x 1.5 mL• DMSO, 1 x 500 μLStore at -20°C.
For Use With (Application):
Cloning, Hot-start PCR
Product Type:
Hot Start High-Fidelity DNA Polymerase
Indicator:
__
Quantity:
__
Cell Permeability: __
Detection Method: __
For Use With (Equipment): __
Dye Type: __
Indicator Properties: __
Shipping Condition: Wet or Dry Ice
Content And Storage: • Phire Hot Start II DNA Polymerase, 1 x 200 μL• 5X Phire Green Reaction Buffer, 2 x 1.5 mL• DMSO, 1 x 500 μLStore at -20°C.
For Use With (Application): Cloning, Hot-start PCR
Product Type: Hot Start High-Fidelity DNA Polymerase
Indicator: __
Quantity: __
Cell Permeability:
__
Detection Method:
__
For Use With (Equipment):
__
Dye Type:
__
Indicator Properties:
__
Shipping Condition:
Wet or Dry Ice
Content And Storage:
• Phire Hot Start II DNA Polymerase, 1 x 200 μL• 5X Phire Green Reaction Buffer, 2 x 1.5 mL• DMSO, 1 x 500 μLStore at -20°C.
For Use With (Application):
Cloning, Hot-start PCR
Product Type:
Hot Start High-Fidelity DNA Polymerase
Indicator:
__
Quantity:
__
Cell Permeability: __
Detection Method: __
For Use With (Equipment): __
Dye Type: __
Indicator Properties: __
Shipping Condition: Wet or Dry Ice
Content And Storage: • 2X Phire Hot Start II PCR Master Mix, 20 x 1.25 mL• DMSO, 4 x 0.5 mL• Nuclease-free water, 20 x 1.25 mLStore at -20°C.
For Use With (Application): Cloning, Hot-start PCR
Product Type: Hot Start PCR Master Mix
Indicator: __
Quantity: __
Cell Permeability:
__
Detection Method:
__
For Use With (Equipment):
__
Dye Type:
__
Indicator Properties:
__
Shipping Condition:
Wet or Dry Ice
Content And Storage:
• 2X Phire Hot Start II PCR Master Mix, 20 x 1.25 mL• DMSO, 4 x 0.5 mL• Nuclease-free water, 20 x 1.25 mLStore at -20°C.
For Use With (Application):
Cloning, Hot-start PCR
Product Type:
Hot Start PCR Master Mix
Indicator:
__
Quantity:
__