A12217

Invitrogen™ Amplex™ Acetylcholine/Acetlycholinesterase Assay Kit

Manufacturer: Invitrogen™

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Pack Size SKU Availability Price
Each of 1 A12217-Each-of-1 In Stock ₹ 37,903.08

A12217 - Each of 1

₹ 37,903.08

In Stock

Quantity

1

Base Price: ₹ 37,903.08

GST (18%): ₹ 6,822.554

Total Price: ₹ 44,725.634

Content And Storage

Store in freezer -5°C to -30°C and protect from light.

For Use With (Application)

Acetylcholine/Acetlycholinesterase Assay

Product Type

Acetylcholine⁄Acetlycholinesterase Assay

Quantity

500 assays

Substrate Properties

Molecular Substrate

Target Enzyme

Acetylcholinesterase

Detection Method

Fluorescence Intensity

For Use With (Equipment)

Fluorescence Microplate Reader

Product Line

Amplex™

Shipping Condition

Room Temperature

Substrate Type

HRP (Horseradish Peroxidase) Substrate, Acetylcholinesterase Substrate

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Description

  • The Amplex Red Acetylcholine/Acetylcholinesterase Assay Kit provides an ultrasensitive method for detecting acetylcholinesterase (AChE) activity in a fluorescence microplate reader or fluorometer
  • AChE activity is monitored indirectly using 10-acetyl-3,7-dihydroxyphenoxazine (Amplex Red reagent), a sensitive fluorogenic probe for hydrogen peroxide
  • AChE converts the acetylcholine substrate to choline, which is then oxidized by choline oxidase to betaine and hydrogen peroxide
  • In the presence of horseradish peroxidase, hydrogen peroxide reacts with the Amplex Red reagent in a 1:1 stoichiometric ratio to generate the highly fluorescent product resorufin
  • Little interference from autofluorescence in most biological samples Detect AChE activity levels as low as 0.002U/mL in one hour Detect acetylcholine levels as low as 0.3μM using excess AChE Achieve detection ranges of 0.3μM to 100μM acetylcholine Format allows for multiple time point measurements Designed for minimal autofluorescence interference Custom assay design and packaging are also available Drug Discovery and Development, Enzyme and Protein Activity Assays, Proteins, Expression, Isolation and Analysis, Target and Lead Identification and Validation Order Info Shipping Condition: Room temperature

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Invitrogen™

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Content And Storage:
Store in freezer -5°C to -30°C and protect from light.

For Use With (Application):
Acetylcholine/Acetlycholinesterase Assay

Product Type:
Acetylcholine⁄Acetlycholinesterase Assay

Quantity:
500 assays

Substrate Properties:
Molecular Substrate

Target Enzyme:
Acetylcholinesterase

Detection Method:
Fluorescence Intensity

For Use With (Equipment):
Fluorescence Microplate Reader

Product Line:
Amplex™

Shipping Condition:
Room Temperature

Substrate Type:
HRP (Horseradish Peroxidase) Substrate, Acetylcholinesterase Substrate

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Room Temperature

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Fluorometer, Microplate Reader

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Room Temperature

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