A12218
Invitrogen™ Amplex™ Red Phosphatidylcholine-Specific Phospholipase C Assay Kit
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | A12218-Each-of-1 | In Stock | ₹ 40,139.00 |
A12218 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 40,139.00
GST (18%): ₹ 7,225.02
Total Price: ₹ 47,364.02
Content And Storage
Store in freezer -5°C to -30°C and protect from light.
For Use With (Application)
Phospholipase Assay
Product Type
Red Phosphatidylcholine-Specific Phospholipase C Assay Kit
Format
Cuvettes, 96-well plate
Quantity
500 assays
Detection Method
Fluorescence
For Use With (Equipment)
Fluorometer, Microplate Reader
Dye Type
Other Label(s) or Dye(s)
Product Line
Amplex™
Shipping Condition
Room Temperature
Related Products
Description
- The Amplex Red Phosphatidylcholine-Specific Phospholipase C Assay Kit provides a sensitive method for detecting phosphatidylcholine-specific phospholipase C (PC-PLC) activity using a fluorescence microplate reader or fluorometer
- In this enzyme-coupled assay, PC-PLC activity is monitored indirectly using 10-acetyl-3,7-dihydroxyphenoxazine (Amplex Red reagent), a sensitive fluorogenic probe for hydrogen peroxide
- PC-PLC converts phosphatidylcholine (lecithin) to phosphocholine and diacylglycerol
- Alkaline phosphatase is added, which hydrolyzes phosphorylcholine to form choline
- Choline is then oxidized by choline oxidase to form betaine and hydrogen peroxide
- In the presence of horseradish peroxidase, the hydrogen peroxide reacts with Amplex Red reagent in a 1:1 stoichiometric ratio to generate the highly fluorescent product resorufin
- Because resorufin has absorption and fluorescence emission maxima of approximately 571nm and 585nm, respectively, there is little interference from autofluorescence in most biological samples
- Detects phospholipase-C activity levels as low as 0.2mU/mL Format allows for multiple time point measurements Designed for minimal autofluorescence interference Custom assay design and packaging are also available Use Amplex Red Assays for a Broad Range of Investigations A wide variety of validated Amplex Red assays are available for studying cell signaling and lipids, neurobiology, inflammation and immune function, and metabolism
- We also offer Amplex UltraRed Reagent, a second-generation reagent providing greater sensitivity and brighter fluorescence, and the Amplex Red/UltraRed Stop Reagent
- The Amplex Red/UltraRed Stop Reagent provides convenience and control by allowing the fluorescence signal-generating reaction to be terminated at a user-determined time point
- After addition of the stop reagent, the fluorescence signal remains stable for at least three hours
- Enzyme and Protein Activity Assays, Lipase and Phopholipase Activity, Proteins, Expression, Isolation and Analysis Order Info Shipping Condition: Room temperature
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Content And Storage: Store in freezer -5°C to -30°C and protect from light.
For Use With (Application): Phospholipase Assay
Product Type: Red Phosphatidylcholine-Specific Phospholipase C Assay Kit
Format: Cuvettes, 96-well plate
Quantity: 500 assays
Detection Method: Fluorescence
For Use With (Equipment): Fluorometer, Microplate Reader
Dye Type: Other Label(s) or Dye(s)
Product Line: Amplex™
Shipping Condition: Room Temperature
Content And Storage:
Store in freezer -5°C to -30°C and protect from light.
For Use With (Application):
Phospholipase Assay
Product Type:
Red Phosphatidylcholine-Specific Phospholipase C Assay Kit
Format:
Cuvettes, 96-well plate
Quantity:
500 assays
Detection Method:
Fluorescence
For Use With (Equipment):
Fluorometer, Microplate Reader
Dye Type:
Other Label(s) or Dye(s)
Product Line:
Amplex™
Shipping Condition:
Room Temperature
Content And Storage: Store in freezer -5°C to -30°C and protect from light.
For Use With (Application): Sphingomyelinase Assay
Product Type: Red Sphingomyelinase Assay Kit
Format: 96-well plate
Quantity: 500 assays
Detection Method: Fluorescence
For Use With (Equipment): Fluorometer, Microplate Reader
Dye Type: Other Label(s) or Dye(s)
Product Line: Amplex™
Shipping Condition: Wet Ice
Content And Storage:
Store in freezer -5°C to -30°C and protect from light.
For Use With (Application):
Sphingomyelinase Assay
Product Type:
Red Sphingomyelinase Assay Kit
Format:
96-well plate
Quantity:
500 assays
Detection Method:
Fluorescence
For Use With (Equipment):
Fluorometer, Microplate Reader
Dye Type:
Other Label(s) or Dye(s)
Product Line:
Amplex™
Shipping Condition:
Wet Ice
Content And Storage: Store in freezer -5°C to -30°C and protect from light.
For Use With (Application): Glutamic Acid/Glutamate Oxidase Assay
Product Type: Red Glutamic Acid/Glutamate Oxidase Assay Kit
Format: 96-well plate
Quantity: 200 assays
Detection Method: Fluorescence
For Use With (Equipment): Fluorometer, Microplate Reader
Dye Type: Other Label(s) or Dye(s)
Product Line: Amplex™
Shipping Condition: Room Temperature
Content And Storage:
Store in freezer -5°C to -30°C and protect from light.
For Use With (Application):
Glutamic Acid/Glutamate Oxidase Assay
Product Type:
Red Glutamic Acid/Glutamate Oxidase Assay Kit
Format:
96-well plate
Quantity:
200 assays
Detection Method:
Fluorescence
For Use With (Equipment):
Fluorometer, Microplate Reader
Dye Type:
Other Label(s) or Dye(s)
Product Line:
Amplex™
Shipping Condition:
Room Temperature
Content And Storage: Store in freezer (-5°C to -30°C).
For Use With (Application): Hydrogen Peroxidase Assay
Product Type: Red Hydrogen Peroxide
Format: __
Quantity: 5 mg
Detection Method: Fluorescence
For Use With (Equipment): Fluorometer, Microplate Reader
Dye Type: __
Product Line: Amplex™
Shipping Condition: Room Temperature
Content And Storage:
Store in freezer (-5°C to -30°C).
For Use With (Application):
Hydrogen Peroxidase Assay
Product Type:
Red Hydrogen Peroxide
Format:
__
Quantity:
5 mg
Detection Method:
Fluorescence
For Use With (Equipment):
Fluorometer, Microplate Reader
Dye Type:
__
Product Line:
Amplex™
Shipping Condition:
Room Temperature