Invitrogen™ Amplex™ Red Glutamic Acid/Glutamate Oxidase Assay Kit

Description

• The Amplex Red Glutamic Acid/Glutamate Oxidase Assay Kit provides a sensitive and simple method for detecting glutamic acid/glutamate oxidase activity using a fluorescence microplate reader or fluorometer
• The Amplex Red Glutamic Acid/Glutamate Oxidase Assay Kit provides an ultrasensitive method for detecting glutamic acid or for continuously monitoring glutamate oxidase activity using a fluorescence microplate reader or fluorometer
• In the assay, L-glutamic acid is oxidized by glutamate oxidase to produce α-ketoglutarate, NH 3 , and hydrogen peroxide
• L-alanine and L-glutamate–pyruvate transaminase are included in the reaction to regenerate L-glutamic acid by transamination of α-ketoglutarate, resulting in multiple cycles of the initial reaction and a significant amplification of the hydrogen peroxide produced
• The hydrogen peroxide reacts with 10-acetyl-3,7-dihydroxyphenoxazine (Amplex Red reagent) in a 1:1 stoichiometric ratio in a reaction catalyzed by horseradish peroxidase (HRP) to generate the highly fluorescent product resorufin
• Because resorufin has absorption and fluorescence emission maxima of approximately 571nm and 585nm, respectively, there is little interference from autofluorescence in most biological samples
• Detect concentrations as low as 10nm L-glutamic acid or 40μU/mL of purified L-glutamate oxidase Format allows for multiple time point measurements Designed for minimal autofluorescence interference Custom assay design and packaging are also available Use Amplex Red Assays for a Broad Range of Investigations A wide variety of validated Amplex Red assays are available for studying cell signaling and lipids, neurobiology, inflammation and immune function, and metabolism
• We also offer Amplex UltraRed Reagent, a second-generation reagent providing greater sensitivity and brighter fluorescence, and the Amplex Red/UltraRed Stop Reagent
• The Amplex Red/UltraRed Stop Reagent provides convenience and control by allowing the fluorescence signal-generating reaction to be terminated at a user-determined time point
• After addition of the stop reagent, the fluorescence signal remains stable for at least three hours
• Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Enzyme and Protein Activity Assays, Oxidase and Peroxidase Activity, Proteins, Expression, Isolation and Analysis Order Info Shipping Condition: Room temperature