A22181

Invitrogen™ Amplex™ Red Uric Acid/Uricase Assay Kit

Manufacturer: Invitrogen™

Select a Size

Pack Size SKU Availability Price
Each of 1 A22181-Each-of-1 In Stock ₹ 38,673.12

A22181 - Each of 1

₹ 38,673.12

In Stock

Quantity

1

Base Price: ₹ 38,673.12

GST (18%): ₹ 6,961.162

Total Price: ₹ 45,634.282

Content And Storage

Store in freezer -5°C to -30°C and protect from light.

For Use With (Application)

Uric Acid/Uricase Assay

Product Type

Red Uric Acid/Uricase Assay Kit

Format

Tube(s), 96-well plate

Quantity

400 assays

Detection Method

Fluorescence

For Use With (Equipment)

Microplate Reader, Spectrophotometer, Fluorometer

Dye Type

Other Label(s) or Dye(s)

Product Line

Amplex™

Shipping Condition

Room Temperature

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Description

  • The Amplex Red Uric Acid/Uricase Assay Kit provides a sensitive and simple method for detecting uric acid or uricase using either a fluorescence microplate reader or fluorometer
  • Serum uric acid is the end product of purine metabolism in the body tissues and is cleared through the kidneys by glomerular filtration
  • Most animals can metabolize uric acid to more readily excreted products, but humans lack the necessary enzyme, urate oxidase (uricase), as a result of the presence of two “nonsense mutations” in the human gene for uricase
  • The Amplex Red Uric Acid/Uricase Assay Kit provides an ultrasensitive method for detecting uric acid or for monitoring uricase activity
  • In the assay, uricase catalyzes the conversion of uric acid to allantoin, hydrogen peroxide, and carbon dioxide
  • In the presence of horseradish peroxidase (HRP), hydrogen peroxide reacts stoichiometrically with the Amplex Red reagent to generate the red-fluorescent oxidation product resorufin
  • Because resorufin has absorption and fluorescence emission maxima of approximately 571nm and 585nm, respectively, there is little interference from autofluorescence in most biological samples
  • Detect concentrations as low as 100nm uric acid or 0.2mU/mL of uricase activity Format allows for multiple time point measurements Designed for minimal autofluorescence interference Custom assay design and packaging are also available Use Amplex Red Assays for a Broad Range of Investigations A wide variety of validated Amplex Red assays are available for studying cell signaling and lipids, neurobiology, inflammation and immune function, and metabolism
  • We also offer Amplex UltraRed Reagent, a second-generation reagent providing greater sensitivity and brighter fluorescence, and the Amplex Red/UltraRed Stop Reagent
  • The Amplex Red/UltraRed Stop Reagent provides convenience and control by allowing the fluorescence signal-generating reaction to be terminated at a user-determined time point
  • After addition of the stop reagent, the fluorescence signal remains stable for at least three hours
  • Enzyme and Protein Activity Assays, Oxidase and Peroxidase Activity, Proteins, Expression, Isolation and Analysis Order Info Shipping Condition: Room temperature

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Content And Storage:
Store in freezer -5°C to -30°C and protect from light.

For Use With (Application):
Uric Acid/Uricase Assay

Product Type:
Red Uric Acid/Uricase Assay Kit

Format:
Tube(s), 96-well plate

Quantity:
400 assays

Detection Method:
Fluorescence

For Use With (Equipment):
Microplate Reader, Spectrophotometer, Fluorometer

Dye Type:
Other Label(s) or Dye(s)

Product Line:
Amplex™

Shipping Condition:
Room Temperature

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Format:
Tube(s), 96-well plate

Quantity:
400 assays

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Fluorescence

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Microplate Reader, Spectrophotometer, Fluorometer

Dye Type:
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Product Line:
Amplex™

Shipping Condition:
Room Temperature

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Store in freezer -5°C to -30°C and protect from light.

For Use With (Application):
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Format:
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Detection Method:
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For Use With (Equipment):
Microplate Reader, Spectrophotometer, Fluorometer

Dye Type:
Other Label(s) or Dye(s)

Product Line:
Amplex™

Shipping Condition:
Room Temperature