Invitrogen™ Amplex™ Red Glucose/Glucose Oxidase Assay Kit

Description

• The Amplex Red Glucose/Glucose Oxidase Assay Kit provides a sensitive and simple method for detecting d-glucose or glucose oxidase using a fluorescence microplate reader or fluorometer
• The Amplex Red reagent (10-acetyl-3,7-dihydroxyphenoxazine) is a colorless, stable, and extremely versatile peroxidase substrate
• Because peroxidase- and glucose oxidase-mediated reactions can be coupled, it is possible to measure glucose oxidase activity or the release of glucose by any glucosidase enzyme—for instance, β-glucosidase and glucocerebrosidase—in either a continuous or discontinuous assay
• Because of the ability to couple reactions, this assay has been demonstrated to be useful for the quantification of glucose levels in foods, fermentation media, and bodily fluids
• In the assay, glucose oxidase reacts with d-glucose to form d-gluconolactone and hydrogen peroxide
• In the presence of horseradish peroxidase (HRP), hydrogen peroxide then reacts with the Amplex Red reagent in a 1:1 stoichiometric ratio to generate the red-fluorescent product resorufin
• Because resorufin has absorption and fluorescence emission maxima of approximately 571nm and 585nm, respectively, there is little interference from autofluorescence in most biological samples
• Detect concentrations as low as 3μM d-glucose or 0.05mU/mL of glucose oxidase Format allows for multiple time point measurements Designed for minimal autofluorescence interference Custom assay design and packaging are also available Use Amplex Red Assays for a Broad Range of Investigations A wide variety of validated Amplex Red assays are available for studying cell signaling and lipids, neurobiology, inflammation and immune function, and metabolism
• We also offer Amplex UltraRed Reagent, a second-generation reagent providing greater sensitivity and brighter fluorescence, and the Amplex Red/UltraRed Stop Reagent
• The Amplex Red/UltraRed Stop Reagent provides convenience and control by allowing the fluorescence signal-generating reaction to be terminated at a user-determined time point
• After addition of the stop reagent, the fluorescence signal remains stable for at least three hours
• Enzyme and Protein Activity Assays, Oxidase and Peroxidase Activity, Proteins, Expression, Isolation and Analysis Order Info Shipping Condition: Room temperature