BD Pharmingen™ 488 EdU Click Proliferation Kit, 50 Tests

Description

• The BD Pharmingen™ 488 EdU Click Proliferation Kit provides a tool for analyzing DNA synthesis in cycling cells
• In this method, the thymidine analog EdU (5-ethynyl-2'-deoxyuridine) is incorporated into newly synthesized DNA by cells progressing through the S (DNA synthesis) phase of the cell cycle
• EdU can then be detected in fixed and permeabilized cells with a fluorophore-labeled azide
• The reaction of the fluorophore-labeled azide and EdU occurs via a copper-catalyzed click reaction between the azide moiety of the fluorophore and the alkyne moiety of EdU
• When co-stained with a DNA dye such as 7-AAD, PI, or DAPI, cell populations may be segmented by flow cytometry into the G0/G1-phases (2N DNA content, EdU-negative), S-phase (2N-4N DNA content, EdU-positive), or G2/M-phases (4N DNA content, EdU-negative)
• The BD Pharmingen™ 488 EdU Click Proliferation Kit contains 6-FAM Azide, which is excited by the blue laser and has an excitation maximum of 496 nm and an emission maximum of 516 nm
• Please note that this kit is provided as Part 1 of 2 (Components A, B, and C) to be stored dry and protected from light at -20°C, and Part 2 of 2 (Components D, E, F, and G) to be stored dry at 2 - 8°C or room temperature.