Invitrogen™ CellLight™ Late Endosomes-GFP, BacMam 2.0

Description

• This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses GFP fused to Rab7a
• You can observe late endosomes-GFP behavior in live cells independently of organelle pH and label with multiple tracking or tracing dyes to image dynamic cellular processes
• Cells expressing CellLight constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques
• BacMam Technology CellLight Late Endosomes-GFP, BacMam 2.0, is a fusion construct of Rab7a and emGFP, providing accurate and specific targeting to cellular Late Endosomes-GFP
• This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories
• BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity
• This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses
• Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting
• CellLight reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution
• Fast and convenient: simply add CellLight reagent to cells, incubate overnight, and image—or store frozen, assay-ready cells for later use Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose Less toxic: CellLight reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling Cell Analysis, Cell Structure, Cell-Based Reporter Assays, Cellular Imaging, Endosomes, Fluorescent Protein Assays (GFP), High-Content Screening (HCS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunofluorescence Staining & Detection, In Vivo Imaging Order Info Shipping Condition: Wet Ice