Invitrogen™ CellLight™ ER-GFP, BacMam 2.0

Description

• This ready-to-use construct is transfected into cells using BacMam 2.0 technology and expresses GFP fused to the ER signal sequence of calreticulin and KDEL (ER retention signal) with minimal cellular disruption
• You can observe ER-GFP behavior in live cells using fluorescent imaging and multiplex with other fluorescent proteins or organic dyes
• Cells expressing CellLight constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques
• BacMam Technology CellLight ER-GFP, BacMam 2.0, is a fusion construct of ER signal sequence of calreticulin and KDEL (ER retention signal) and emGFP, providing accurate and specific targeting to cellular ER-GFP
• This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories
• BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity
• This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses
• Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting
• CellLight reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution
• Fast and convenient: simply add CellLight reagent to cells, incubate overnight, and image—or store frozen, assay-ready cells for later use Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose Less toxic: CellLight reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling Flow Cytometer Laser Lines: 488 Cell Analysis, Cell Structure, Cell Tracing & Tracking, Cellular Imaging, Endoplasmic Reticulum (ER), Immunocytochemistry (ICC), Immunofluorescence (IF), Organelle Tracing Order Info Shipping Condition: Wet Ice