C20300
Invitrogen™ CyQUANT™ LDH Cytotoxicity Assay
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | C20300-Each-of-1 | In Stock | ₹ 15,931.00 |
C20300 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 15,931.00
GST (18%): ₹ 2,867.58
Total Price: ₹ 18,798.58
Cell Permeability
Cell-impermeant
Content And Storage
• Substrate Mixture, 1 vial lyophilizate• Assay Buffer, 1 x 600 μL• Lysis Buffer, 2.5 mL• Stop Solution, 12 mL• LDH Positive Control, 6 μLStore at -5 to -30°C, protect from light
Detection Method
Colorimetric
For Use With (Equipment)
Microplate Reader
Format
96-well plate
Quantity
200 Assays
Color
Red
Description
CyQUANT™ LDH Cytotoxicity Assay
For Use With (Application)
LDH Cytotoxicity Assay
Product Type
LDH Cytotoxicity Assay
Product Line
CyQUANT™
Shipping Condition
Dry Ice
Related Products
Description
- CyQUANT™ LDH Cytotoxicity Assay Kit is a colorimetric assay that provides a simple and reliable method for determining cellular cytotoxicity
- Lactate dehydrogenase (LDH) is a cytosolic enzyme present in many different cell types that is released into the cell culture medium upon damage to the plasma membrane
- The CyQUANT LDH Cytotoxicity Assay Kit provides the reagents to accurately and quantitatively measure this extracellular LDH
- CyQUANT™ LDH Cytotoxicity Assay Kit features include: Convenient —add-mix-read assay format for adherent and suspension cells, including 3D cell models Accurate —provides a quantitative measurement of LDH release Flexible —ideal for high-throughput screening, monitor cytotoxicity from the same sample over time Robust —uses stable LDH enzyme activity as a cytotoxic marker LDH is a cytosolic enzyme present in many different cell types and is a well-established and reliable indicator of cellular toxicity
- Damage of the plasma membrane results in a release of LDH into the surrounding cell culture medium
- This extracellular LDH can be quantified by a coupled enzymatic reaction in which LDH catalyzes the conversion of lactate to pyruvate via NAD+ reduction to NADH
- Diaphorase then uses NADH to reduce a tetrazolium salt (INT) to a red formazan product that can be measured at 490 nm
- The level of formazan formation is directly proportional to the amount of LDH released into the medium
- The CyQUANT™ LDH Cytotoxicity Assay Kit provides the reagents needed for the simple, reliable colorimetric quantification of cellular cytotoxicity
- The kit can be used with different cell types, including 3D cell models, to measure cytotoxicity mediated by chemical compounds as well as cell-mediated cytotoxicity
- Since LDH in the medium is the indicator of cellular cytotoxicity, the assay can be used to monitor cytotoxicity from the same sample over time
- To perform the assay, an aliquot of the cell culture medium is transferred to a new plate and the reaction mixture is added
- After a 30-minute incubation, the reaction is stopped by adding Stop Solution and absorbance is measured using a microplate reader
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Cell Permeability: Cell-impermeant
Content And Storage: • Substrate Mixture, 1 vial lyophilizate• Assay Buffer, 1 x 600 μL• Lysis Buffer, 2.5 mL• Stop Solution, 12 mL• LDH Positive Control, 6 μLStore at -5 to -30°C, protect from light
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Format: 96-well plate
Quantity: 200 Assays
Color: Red
Description: CyQUANT™ LDH Cytotoxicity Assay
For Use With (Application): LDH Cytotoxicity Assay
Product Type: LDH Cytotoxicity Assay
Product Line: CyQUANT™
Shipping Condition: Dry Ice
Cell Permeability:
Cell-impermeant
Content And Storage:
• Substrate Mixture, 1 vial lyophilizate• Assay Buffer, 1 x 600 μL• Lysis Buffer, 2.5 mL• Stop Solution, 12 mL• LDH Positive Control, 6 μLStore at -5 to -30°C, protect from light
Detection Method:
Colorimetric
For Use With (Equipment):
Microplate Reader
Format:
96-well plate
Quantity:
200 Assays
Color:
Red
Description:
CyQUANT™ LDH Cytotoxicity Assay
For Use With (Application):
LDH Cytotoxicity Assay
Product Type:
LDH Cytotoxicity Assay
Product Line:
CyQUANT™
Shipping Condition:
Dry Ice
Cell Permeability: Cell-impermeant
Content And Storage: • Substrate Mixture, 5 vials lyophilizate• Assay Buffer, 5 x 600 μL• Lysis Buffer, 12 mL• Stop Solution, 60 mL• LDH Positive Control, 30 μLStore at -5 to -30°C, protect from light
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Format: 96-well plate
Quantity: 1000 Assays
Color: Red
Description: CyQUANT™ LDH Cytotoxicity Assay
For Use With (Application): LDH Cytotoxicity Assay
Product Type: LDH Cytotoxicity Assay
Product Line: CyQUANT™
Shipping Condition: Dry Ice
Cell Permeability:
Cell-impermeant
Content And Storage:
• Substrate Mixture, 5 vials lyophilizate• Assay Buffer, 5 x 600 μL• Lysis Buffer, 12 mL• Stop Solution, 60 mL• LDH Positive Control, 30 μLStore at -5 to -30°C, protect from light
Detection Method:
Colorimetric
For Use With (Equipment):
Microplate Reader
Format:
96-well plate
Quantity:
1000 Assays
Color:
Red
Description:
CyQUANT™ LDH Cytotoxicity Assay
For Use With (Application):
LDH Cytotoxicity Assay
Product Type:
LDH Cytotoxicity Assay
Product Line:
CyQUANT™
Shipping Condition:
Dry Ice
Cell Permeability: Cell-impermeant
Content And Storage: • 1 vial Reagent Mix• 1 x 12 mL Reporter Mix• 1 x 2.5 mL Lysis Buffer• 1 x 12 mL Stop Solution• 1 x 6 μL LDH Positive ControlStore at -20°C, protect from light.
Detection Method: Fluorescence
For Use With (Equipment): Microplate Reader
Format: 96-well plate
Quantity: 200 Assays
Color: Red
Description: CyQUANT™ LDH Cytotoxicity Assay, fluorescence
For Use With (Application): LDH Cytotoxicity Assay
Product Type: LDH Cytotoxicity Assay
Product Line: CyQUANT™
Shipping Condition: Dry Ice
Cell Permeability:
Cell-impermeant
Content And Storage:
• 1 vial Reagent Mix• 1 x 12 mL Reporter Mix• 1 x 2.5 mL Lysis Buffer• 1 x 12 mL Stop Solution• 1 x 6 μL LDH Positive ControlStore at -20°C, protect from light.
Detection Method:
Fluorescence
For Use With (Equipment):
Microplate Reader
Format:
96-well plate
Quantity:
200 Assays
Color:
Red
Description:
CyQUANT™ LDH Cytotoxicity Assay, fluorescence
For Use With (Application):
LDH Cytotoxicity Assay
Product Type:
LDH Cytotoxicity Assay
Product Line:
CyQUANT™
Shipping Condition:
Dry Ice
Cell Permeability: Cell-impermeant
Content And Storage: • 5 vials Reagent Mix• 5 x 12 mL Reporter Mix• 1 x 12 mL Lysis Buffer• 1 x 60 mL Stop Solution• 1 x 30 μL LDH Positive ControlStore at -20°C, protect from light.
Detection Method: Fluorescence
For Use With (Equipment): Microplate Reader
Format: 96-well plate
Quantity: 1000 Assays
Color: Red
Description: CyQUANT™ LDH Cytotoxicity Assay, fluorescence
For Use With (Application): LDH Cytotoxicity Assay
Product Type: LDH Cytotoxicity Assay
Product Line: CyQUANT™
Shipping Condition: Dry Ice
Cell Permeability:
Cell-impermeant
Content And Storage:
• 5 vials Reagent Mix• 5 x 12 mL Reporter Mix• 1 x 12 mL Lysis Buffer• 1 x 60 mL Stop Solution• 1 x 30 μL LDH Positive ControlStore at -20°C, protect from light.
Detection Method:
Fluorescence
For Use With (Equipment):
Microplate Reader
Format:
96-well plate
Quantity:
1000 Assays
Color:
Red
Description:
CyQUANT™ LDH Cytotoxicity Assay, fluorescence
For Use With (Application):
LDH Cytotoxicity Assay
Product Type:
LDH Cytotoxicity Assay
Product Line:
CyQUANT™
Shipping Condition:
Dry Ice