Invitrogen™ CyQUANT™ LDH Cytotoxicity Assay

Description

• CyQUANT™ LDH Cytotoxicity Assay Kit is a colorimetric assay that provides a simple and reliable method for determining cellular cytotoxicity
• Lactate dehydrogenase (LDH) is a cytosolic enzyme present in many different cell types that is released into the cell culture medium upon damage to the plasma membrane
• The CyQUANT LDH Cytotoxicity Assay Kit provides the reagents to accurately and quantitatively measure this extracellular LDH
• CyQUANT™ LDH Cytotoxicity Assay Kit features include: Convenient —add-mix-read assay format for adherent and suspension cells, including 3D cell models Accurate —provides a quantitative measurement of LDH release Flexible —ideal for high-throughput screening, monitor cytotoxicity from the same sample over time Robust —uses stable LDH enzyme activity as a cytotoxic marker LDH is a cytosolic enzyme present in many different cell types and is a well-established and reliable indicator of cellular toxicity
• Damage of the plasma membrane results in a release of LDH into the surrounding cell culture medium
• This extracellular LDH can be quantified by a coupled enzymatic reaction in which LDH catalyzes the conversion of lactate to pyruvate via NAD+ reduction to NADH
• Diaphorase then uses NADH to reduce a tetrazolium salt (INT) to a red formazan product that can be measured at 490 nm
• The level of formazan formation is directly proportional to the amount of LDH released into the medium
• The CyQUANT™ LDH Cytotoxicity Assay Kit provides the reagents needed for the simple, reliable colorimetric quantification of cellular cytotoxicity
• The kit can be used with different cell types, including 3D cell models, to measure cytotoxicity mediated by chemical compounds as well as cell-mediated cytotoxicity
• Since LDH in the medium is the indicator of cellular cytotoxicity, the assay can be used to monitor cytotoxicity from the same sample over time
• To perform the assay, an aliquot of the cell culture medium is transferred to a new plate and the reaction mixture is added
• After a 30-minute incubation, the reaction is stopped by adding Stop Solution and absorbance is measured using a microplate reader