Invitrogen™ CM-H2DCFDA (General Oxidative Stress Indicator)

Description

• CM-H 2 DCFDA is a chloromethyl derivative of H 2 DCFDA, useful as an indicator for reactive oxygen species (ROS) in cells
• This indicator exhibits much better retention in live cells than H 2 DCFDA
• CM-H 2 DCFDA passively diffuses into cells, where its acetate groups are cleaved by intracellular esterases and its thiol-reactive chloromethyl group reacts with intracellular glutathione and other thiols
• Subsequent oxidation yields a fluorescent adduct that is trapped inside the cell, thus facilitating long-term studies
• ROS Indicator Specifications: • Excitation: ∼492–495 nm • Emission: 517–527 nm • Product is air sensitive and should be stored under dry argon or nitrogen • Product may be dissolved in DMSO, DMF, or ethanol for use • Indicator is cell permeant (cell loading protocols are available in the literature) • Fluorescence can be monitored using a flow cytometer, fluorometer, microplate reader, or fluorescence microscope, using excitation sources and filters appropriate for fluorescein Find more ROS indicators We offer an assortment of Molecular Probes™ products for the generation of reactive oxygen species (ROS), including singlet oxygen, superoxide, hydroxyl radical and various peroxide and hydroperoxides, as well as for their fluorometric detection in solution
• Review Generating and Detecting Reactive Oxygen Species—Section 18.2 in the Molecular Probes™ Handbook for more information on these products
• Dissolve the dye using high-quality, anhydrous dimethylsulfoxide (DMSO), dimethylformamide (DMF), or 100% ethanol to prepare stock concentration up to 5 mM
• Working solutions should be freshly prepared
• This probe oxidizes readily in solution thus presence of moisture will facilitate the decomposition of the dye
• Order Info Shipping Condition: Room Temperature