Invitrogen™ BODIPY™ 581/591 C11 (Lipid Peroxidation Sensor)

Description

• BODIPY™ 581/591 C11 undecanoic acid (Lipid Peroxidation Sensor) is a sensitive fluorescent probe for lipid peroxidation assays that localizes to membranes in live cells
• Oxidation of the polyunsaturated butadienyl portion of this fatty acid analog in live cells results in a shift of the fluorescence emission peak from red (∼590 nm) to green (∼510 nm), allowing ratiometric analysis of lipid peroxidation using fluorescence microscopy, high-content analysis, and flow cytometry
• In the reduced state, the excitation and emission maxima of BODIPY 581/591 C11 is 581/591 nm; after oxidation, the probe shifts the excitation and emission to 488/510 nm
• Lipid peroxidation is the oxidative degradation of cellular lipids by free radicals from reactive oxygen species, which can degrade lipids containing carbon-carbon double bonds such as phospholipids and polyunsaturated fatty acids
• Oxidation of lipids leads to the generation of lipid peroxides and can cause damage to cell membranes, resulting in changes to signal transduction pathways and eventually leading to cell death
• Lipid peroxidation is involved in apoptosis and one of the main contributors to ferroptosis, an iron-dependent, non-apoptotic form of cell death
• Oxidative stress from lipid peroxidation plays a role in aging, as well as pathologies such as cancer, atherosclerosis, and neurodegenerative diseases
• The BODIPY 581/591 C11 reagent provides a sensitive method to measure lipid peroxidation that occurs in these biological processes and pathological conditions
• Because it shifts its fluorescence emission upon oxidation and can be measured through ratiometric fluorescence analysis at two wavelengths, measurement with the BODIPY 581/591 C11 reagent can minimize variations in fluorescence intensity due to factors such as indicator concentration, excitation intensity, and photobleaching
• The BODIPY 581/591 C11 reagent can also be used for fluorometric lipid peroxidation assays of antioxidant efficacy in plasma and lipid vesicles
• The BODIPY™ 581/591 C11 (Lipid Peroxidation Sensor) reagent is also available in the Image-iT™ Lipid Peroxidation Kit (Cat
• No
• C10445) which includes cumene hydroperoxide as a positive control to induce lipid peroxidation in live cells
• We recommend dissolving in high-quality anhydrous DMSO to attain a stock concentration of 1 to 10 mM
• Order Info Shipping Condition: Room Temperature