K1636
Thermo Scientific™ CellSensor™ CRE-blaFreeStyle™ 293F Cell Line
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K1636-Each-of-1 | In Stock | ₹ 9,05,224.80 |
K1636 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 9,05,224.80
GST (18%): ₹ 1,62,940.464
Total Price: ₹ 10,68,165.264
Assay Entry
Cell-based beta lactamase reporter gene
Content And Storage
CellSensor™ Cell Lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
Quantity
1 Vial
System Type
CellSensor™
Cell State
Dividing Cells
Product Line
CellSensor™
Target Entry
PKA, cAMP, cAMP/PKA pathway
Druggable Target
Signaling Pathway
Related Products
Description
- The CellSensor™ CRE-bla FreeStyle™ 293F cell line contains a beta-lactamase reporter gene under control of the CRE response element stably integrated into FreeStyle™ 293F cells
- To construct this cell line, the CRE -bla construct was transduced into FreeStyle™ 293F cells by lentivirus
- Flow cytometry was used to isolate cells responsive to forskolin
- Assay development experiments performed on this cell line included DMSO tolerance, cell number, stimulation time and substrate loading time
- Functional validation of this cell line under optimized conditions included and Z' and EC50 determinations using forskolin as a primary agonist
- The CellSensor™ CRE-bla FreeStyle™ 293F cell line is responsive to forskolin and can be used to build specific G-protein coupled receptor (GPCR) assays after transfection of additional genes of interest or to detect changes in cellular cAMP levels
- This cell line can be adapted for high-throughput screening for agonists or antagonists with compound libraries
- Candidate drugs can be tested for dose response against this cell line
- Academic and non-profit customers, please inquire for special pricing.
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Assay Entry: Cell-based beta lactamase reporter gene
Content And Storage: CellSensor™ Cell Lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
Quantity: 1 Vial
System Type: CellSensor™
Cell State: Dividing Cells
Product Line: CellSensor™
Target Entry: PKA, cAMP, cAMP/PKA pathway
Druggable Target: Signaling Pathway
Assay Entry:
Cell-based beta lactamase reporter gene
Content And Storage:
CellSensor™ Cell Lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
Quantity:
1 Vial
System Type:
CellSensor™
Cell State:
Dividing Cells
Product Line:
CellSensor™
Target Entry:
PKA, cAMP, cAMP/PKA pathway
Druggable Target:
Signaling Pathway
Assay Entry: __
Content And Storage: __
Quantity: __
System Type: __
Cell State: __
Product Line: __
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Assay Entry: Cell-based beta lactamase reporter gene
Content And Storage: CellSensor™ Cell Lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
Quantity: 1 Vial
System Type: CellSensor™
Cell State: Dividing Cells
Product Line: CellSensor™
Target Entry: p53, DNA damage/p53 response pathway
Druggable Target: Signaling Pathway
Assay Entry:
Cell-based beta lactamase reporter gene
Content And Storage:
CellSensor™ Cell Lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
Quantity:
1 Vial
System Type:
CellSensor™
Cell State:
Dividing Cells
Product Line:
CellSensor™
Target Entry:
p53, DNA damage/p53 response pathway
Druggable Target:
Signaling Pathway