Invitrogen™ LIVE/DEAD™BacLight™ Bacterial Viability Kits

Description

• The LIVE/DEAD Bac Light Bacterial Viability Kits are convenient and easy-to-use kits for monitoring the viability of bacterial populations based on the membrane integrity of the cell
• These kits provide a two-color fluorescence assay of bacterial viability for a diverse array of bacterial genera
• Cells with a compromised membrane that are dead or dying will stain red with propidium iodide, whereas cells with an intact membrane will stain green with SYTO™ 9
• These kits enable researchers to differentiate between live and dead bacteria within minutes, even in mixed populations with diverse bacterial types through fluorescent imaging, flow cytometry, and microplate assays
• The LIVE/DEAD Bac Light Bacterial Viability Kits utilize mixtures of our SYTO™ 9 green-fluorescent nucleic acid stain and the red-fluorescent nucleic acid stain, propidium iodide
• These stains differ both in their spectral characteristics and in their ability to penetrate healthy bacterial cells
• When used alone, the SYTO 9 stain generally labels all bacteria in a population — those with intact membranes and those with damaged membranes
• In contrast, propidium iodide penetrates only bacteria with damaged membranes, causing a reduction in the SYTO 9 stain fluorescence when both dyes are present
• The LIVE/DEAD Bac Light Bacterial Viability Kits have been thoroughly tested with a variety of organisms and under several conditions
• The kits are ideal for fluorescence microscopy as the live (green fluorescent) and dead (red fluorescent) cells may be viewed separately
• They are also suitable for quantitative analysis with a fluorometer, fluorescence microplate reader, flow cytometer, or other instrumentation
• Traditional methods of assessing bacterial viability rely on metabolic characteristics or membrane integrity
• However, due to differences in morphology, cytology, and physiology among the many bacterial genera, developing a universally applicable direct-count viability assay has been challenging
• The LIVE/DEAD Bac Light Bacterial Viability Kits based on membrane integrity allow researchers to distinguish live and dead bacteria easily, reliably, and quantitatively in minutes, even in a mixed population with diverse bacterial types
• The LIVE/DEAD Bac Light Bacterial Viability Kit is available in three different formats: LIVE/DEAD Bac Light Bacterial Viability Kit, for microscopy (Cat
• No
• L7007) The LIVE/DEAD Bac Light Bacterial Viability Kit, for microscopy includes two different premixed solutions of the SYTO 9 and propidium iodide (PI) in 300 μL of DMSO
• The fluorescent dyes are mixed at different proportions in each one: Component A – 1.67 mM SYTO 9 & 1.67 mM PI, Component B – 1.67 mM STYO 9 & 18.3 mM PI
• This kit is available for customers who have already developed protocols using the premixed dye formulation
• LIVE/DEAD Bac Light Bacterial Viability Kit, for microscopy & quantitative assays (Cat
• No
• L7012) The LIVE/DEAD Bac Light Bacterial Viability Kit, for microscopy & quantitative assays includes two separate solutions of the SYTO 9 (3.34 mM) and propidium iodide (20 mM) dyes in 300 μL of DMSO
• The separate staining components enables flexibility for optimization and facilitates the calibration of bacterial fluorescence for quantitative procedures
• LIVE/DEAD Bac Light Bacterial Viability Kit (Cat
• No
• L13152) For added convenience, the LIVE/DEAD Bac Light Bacterial Viability Kit contains 10 pairs of the premeasured separate SYTO 9 and propidium iodide dyes in sealed polyethylene transfer pipets
• In addition to the convenient packaging in the sealed pipets, the fluorescent dyes are supplied dry, without DMSO or other solvents
• This formulation does not require refrigeration and remains stable at temperatures ≤–20°C once reconstituted
• All three kits come with 10mL of Bac Light mounting oil for bacteria immobilized on membranes
• The refractive index at 25°C is 1.517 ± 0.003
• Any physiological buffer between pH 7.0–8.0, including PBS, can be used to dilute the SYTO dyes for the staining solution
• At the recommended reagent dilutions and volumes, kits L7007 and L7012 contain sufficient material to perform ≥1000 individual tests in 96-well assay plates, many more tests by fluorescence microscopy or ~200 tests by flow cytometry
• In kit L13152, each applicator pair contains sufficient dye to perform 50 individual tests in a 96-well assay plate, ~1000 assays by fluorescence microscopy or 10 tests by flow cytometry
• The LIVE/DEAD Bac Light Bacterial Viability Kits have been tested on the following bacterial species: Bacillus cereus, B
• subtilis, Clostridium perfringens, Escherichia coli, Klebsiella pneumoniae, Micrococcus luteus, Mycobacterium phlei, Pseudomonas aeruginosa, P
• syringae, Salmonella oranienburg, Serratia marcescens, Shigella sonnei, Staphylococcus aureus and Streptococcus pyogenes
• All these bacterial types have shown a good correlation between the results obtained with the LIVE/DEAD Bac Light Bacterial Viability Kits and those obtained with standard plate counts
• These tests were performed on logarithmically growing cultures of organisms
• In addition, we have received favorable reports from researchers who have used these kits with: Agrobacterium tumefaciens, Edwardsiella ictaluri, Eurioplasma eurilytica, Lactobacillus sp., Mycoplasma hominus, Propionibacterium sp., Proteus mirabilis and Zymomonas sp.