Molecular Probes™ Premo™ Halide Sensor

Description

• The Premo™ Halide Sensor is based on a yellow fluorescent protein (YFP) molecule sensitive to halide ions
• The combination of the YFP (Venus) biosensor with efficient and non-cytopathic BacMam delivery and expression gives researchers a highly sensitive, robust and easy-to-use tool to efficiently screen halide ion channels and transporter modulators in their cellular models of choice
• No specialized instruments are needed, as the sensor works on standard HTS platforms
• - Cl– channel–specific—measure halide ion flux in voltage- and ligand-gated chloride channels and transporters - Fast—measure chloride flux in high-throughput mode with highly reproducible results - Robust—reliably high expression of the bright and fast maturating Venus sensor gives an excellent signal window - Convenient—study chloride flux in your cellular model by efficient and non-cytopathic delivery of Premo™ Halide Sensor by BacMam technology - Pharmacologically relevant—known modulators show dose-dependent quenching and BacMam delivery enables assays in primary cells The assay combines the YFP Venus halide sensor with a surrogate ion for chloride (iodide); upon stimulation of the chloride channel or transporter, iodide ions flow down the concentration gradient into the cells and quench YFP fluorescence upon binding; the amount of quench is directly proportional to the ion flux (chloride channel or transporter activity)
• Due to the bright Venus fluorescence, the Premo™ Halide Sensor signal window is excellent
• We have demonstrated the utility and efficiency of the Premo™ Halide Sensor as a robust and true “no wash” HTS chloride assay
• Premo™ Halide Sensor is pre-packaged and ready for immediate use
• It contains all components required for cellular delivery and expression
• Screening can be conducted in complete medium and without any wash steps
• To uncouple cell maintenance and preparation from screening, simply freeze transduced cells and bring out as little as 4 h before the screen
• Both stable cell lines and human primary cells can be prepared frozen and “assay-ready”.