PI75707
Thermo Scientific™ Mammalian beta-Galactosidase Assay Kit
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI75707-Each-of-1 | In Stock | ₹ 35,935.20 |
PI75707 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 35,935.20
GST (18%): ₹ 6,468.336
Total Price: ₹ 42,403.536
Content And Storage
Sufficient For: 250 microplate wells of adherent cellsPierce beta-Galactosidase Assay Reagent, 25 mLM-PER Mammalian Protein Extraction Reagent, 25 mLStop Solution, 25 mLStore the β-Galactosidase Assay Reagent at -20°C. Store all other components at room temperature.
Detection Method
Reporter Enzyme
For Use With (Equipment)
Microplate Reader
Dye Type
Enzyme Labeled
Product Line
Pierce™
Substrate
Proprietary
Sufficient For
250 microplate wells of adherent cells
Description
Pierce Mammalian beta-Galactosidase Assay Kit
For Use With (Application)
Absorbance
Product Type
Beta-Galactosidase Assay Kit
Format
96-well Plate
Quantity
75 mL Kit
Substrate Type
Beta-Gal Substrate
Target
Beta-Galactosidase
Related Products
Description
- Perform a simple colorimetric method for lysing cultured mammalian cells and measuring beta-Gal activity using Thermo Scientific™ Mammalian β-Galactosidase Assay Kit
- The Assay Reagent and Kit comprise a simple and versatile assay system for measuring β-galactosidase activity
- Mammalian cells grown on a plate may be lysed and tested for β-galactosidase activity by adding a single, all-in-one lysis-and-assay solution
- Alternatively, the included Thermo Scientific M-PER™ Mammalian Protein Extraction Reagent may be used to prepare lysate from washed adherent or pelleted cells, and then a portion of the lysate used to quantitate the β-Gal activity
- With M-PER Reagent, there is no need to scrape cells or to perform freeze-thaw cycles
- The remaining lysates prepared with M-PER Reagent may be used for applications such as reporter assays (luciferase, β-galactosidase and chloramphenical acetyltransferase), protein kinase assays (PKA, PKC, and Tyrosine Kinase), immunoassays (Western blot, ELISA, RIA) and protein purification
- Highlights: Choose stand-alone assay reagent or complete kit for cell lysis and detection Colorimetric assay easily measured in a plate reader or spectrophotometer (405nm) Adaptable to 96-well microplates or tube assay formats Perform homogeneous screening assays or measure activity secondarily after cell lysis Also compatiible with prepared lysates from other species such as bacteria Includes: Stand-alone Thermo Scientific Pierce™ β-Galactosidase Assay Reagent, Thermo Scientific M-PER Mammalian Protein Extraction Reagent (Part No
- 78503), and ready-to-use Stop Solution
Compare Similar Items
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Content And Storage: Sufficient For: 250 microplate wells of adherent cellsPierce beta-Galactosidase Assay Reagent, 25 mLM-PER Mammalian Protein Extraction Reagent, 25 mLStop Solution, 25 mLStore the β-Galactosidase Assay Reagent at -20°C. Store all other components at room temperature.
Detection Method: Reporter Enzyme
For Use With (Equipment): Microplate Reader
Dye Type: Enzyme Labeled
Product Line: Pierce™
Substrate: Proprietary
Sufficient For: 250 microplate wells of adherent cells
Description: Pierce Mammalian beta-Galactosidase Assay Kit
For Use With (Application): Absorbance
Product Type: Beta-Galactosidase Assay Kit
Format: 96-well Plate
Quantity: 75 mL Kit
Substrate Type: Beta-Gal Substrate
Target: Beta-Galactosidase
Content And Storage:
Sufficient For: 250 microplate wells of adherent cellsPierce beta-Galactosidase Assay Reagent, 25 mLM-PER Mammalian Protein Extraction Reagent, 25 mLStop Solution, 25 mLStore the β-Galactosidase Assay Reagent at -20°C. Store all other components at room temperature.
Detection Method:
Reporter Enzyme
For Use With (Equipment):
Microplate Reader
Dye Type:
Enzyme Labeled
Product Line:
Pierce™
Substrate:
Proprietary
Sufficient For:
250 microplate wells of adherent cells
Description:
Pierce Mammalian beta-Galactosidase Assay Kit
For Use With (Application):
Absorbance
Product Type:
Beta-Galactosidase Assay Kit
Format:
96-well Plate
Quantity:
75 mL Kit
Substrate Type:
Beta-Gal Substrate
Target:
Beta-Galactosidase
Content And Storage: Store at -20°C.
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Dye Type: __
Product Line: Pierce™
Substrate: Beta-Gal Substrate
Sufficient For: __
Description: __
For Use With (Application): Absorbance
Product Type: Pierce beta-Galactosidase Assay Reagent
Format: 96-well Plate
Quantity: 3 x 25 mL
Substrate Type: __
Target: __
Content And Storage:
Store at -20°C.
Detection Method:
Colorimetric
For Use With (Equipment):
Microplate Reader
Dye Type:
__
Product Line:
Pierce™
Substrate:
Beta-Gal Substrate
Sufficient For:
__
Description:
__
For Use With (Application):
Absorbance
Product Type:
Pierce beta-Galactosidase Assay Reagent
Format:
96-well Plate
Quantity:
3 x 25 mL
Substrate Type:
__
Target:
__
Content And Storage: Sufficient For: 250 colonies or 250 culture samples (100 μL each)Y-PER Yeast Protein Extraction Reagent, 25 mL (store at room temperature)2X beta-Galactosidase Assay Buffer, 25 mL (store at -20°C)1M Sodium Carbonate Stop Solution, 25 mL (store at room temperature)
Detection Method: Reporter Enzyme
For Use With (Equipment): Microplate Reader
Dye Type: Enzyme Labeled
Product Line: Pierce™
Substrate: ONPG
Sufficient For: 250 colonies or 250 culture samples (100 μL each)
Description: __
For Use With (Application): Absorbance
Product Type: Yeast beta-Galactosidase Assay Kit
Format: 96-well Plate
Quantity: 75 mL
Substrate Type: Beta-Gal Substrate
Target: Beta-Galactosidase
Content And Storage:
Sufficient For: 250 colonies or 250 culture samples (100 μL each)Y-PER Yeast Protein Extraction Reagent, 25 mL (store at room temperature)2X beta-Galactosidase Assay Buffer, 25 mL (store at -20°C)1M Sodium Carbonate Stop Solution, 25 mL (store at room temperature)
Detection Method:
Reporter Enzyme
For Use With (Equipment):
Microplate Reader
Dye Type:
Enzyme Labeled
Product Line:
Pierce™
Substrate:
ONPG
Sufficient For:
250 colonies or 250 culture samples (100 μL each)
Description:
__
For Use With (Application):
Absorbance
Product Type:
Yeast beta-Galactosidase Assay Kit
Format:
96-well Plate
Quantity:
75 mL
Substrate Type:
Beta-Gal Substrate
Target:
Beta-Galactosidase
Content And Storage: Store membranes flat at ambient temperature, away from chemical vapors. Some solvent vapors may partially dissolve the membranes, which will disrupt the pore structure. Keep membranes out of direct sunlight. Sunlight may cause the nitrocellulose to dry and become brittle. Note: Nitrocellulose membrane filters are highly flammable. Keep away from heat and open flame. Flash point is approximately 2°C. Membranes can be sterilized by steam-autoclaving at 121°C.
Detection Method: __
For Use With (Equipment): __
Dye Type: __
Product Line: __
Substrate: __
Sufficient For: 25 Mini-gel Blots
Description: Pierce Nitrocellulose Membrane, 0.45 μm, 12 x 8 cm
For Use With (Application): __
Product Type: __
Format: Sheet
Quantity: 25 Pre-cut Membranes
Substrate Type: __
Target: __
Content And Storage:
Store membranes flat at ambient temperature, away from chemical vapors. Some solvent vapors may partially dissolve the membranes, which will disrupt the pore structure. Keep membranes out of direct sunlight. Sunlight may cause the nitrocellulose to dry and become brittle. Note: Nitrocellulose membrane filters are highly flammable. Keep away from heat and open flame. Flash point is approximately 2°C. Membranes can be sterilized by steam-autoclaving at 121°C.
Detection Method:
__
For Use With (Equipment):
__
Dye Type:
__
Product Line:
__
Substrate:
__
Sufficient For:
25 Mini-gel Blots
Description:
Pierce Nitrocellulose Membrane, 0.45 μm, 12 x 8 cm
For Use With (Application):
__
Product Type:
__
Format:
Sheet
Quantity:
25 Pre-cut Membranes
Substrate Type:
__
Target:
__