PI75768
Thermo Scientific™ Yeast beta-Galactosidase Assay Kit
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI75768-Each-of-1 | In Stock | ₹ 27,379.20 |
PI75768 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 27,379.20
GST (18%): ₹ 4,928.256
Total Price: ₹ 32,307.456
Content And Storage
Sufficient For: 250 colonies or 250 culture samples (100 μL each)Y-PER Yeast Protein Extraction Reagent, 25 mL (store at room temperature)2X beta-Galactosidase Assay Buffer, 25 mL (store at -20°C)1M Sodium Carbonate Stop Solution, 25 mL (store at room temperature)
For Use With (Application)
Absorbance
Product Type
Yeast beta-Galactosidase Assay Kit
Format
96-well Plate
Quantity
75 mL
Substrate Type
Beta-Gal Substrate
Target
Beta-Galactosidase
Detection Method
Reporter Enzyme
For Use With (Equipment)
Microplate Reader
Dye Type
Enzyme Labeled
Product Line
Pierce™
Substrate
ONPG
Sufficient For
250 colonies or 250 culture samples (100 μL each)
Related Products
Description
- Thermo Scientific™ Yeast β-Galactosidase Assay Kit is specially formulated for yeast cells and ideal for identifying protein interactions in vivo using two-hybrid systems
- The Yeast β-Galactosidase Assay Kit allows for qualitative or quantitative determination of β-galactosidase activity in solution directly from colonies growing on solid medium
- A portion of the colony is suspended in a mixture of Thermo Scientific Y-PER™ Yeast Protein Extraction Reagent and the β-Galactosidase Assay Buffer
- After a brief incubation period, the solution turns yellow from the hydrolysis of o-nitrophenyl-β-D-galactopyranoside (ONPG) to o-nitrophenol (ONP) and galactose in a mildly alkaline solution
- The assay becomes quantitative if calibrated to cell number by measuring the optical density at 660nm
- Highlights: Efficient lysis of yeast cells and a colorimetric detection system Quantitative or qualitative assay for yeast colonies grown on solid media or in suspension Colorimetric assay easily measured in a plate reader or spectrophotometer (420nm) Adaptable to 96-well microplates or tube assay formats Perform homogeneous screening assays or measure activity secondarily after cell lysis Also compatible with prepared lysates from other species such as bacteria Includes: Thermo Scientific Y-PER Yeast Protein Extraction Reagent (Part No
- 78990), 2X Assay Buffer and Stop Solution
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Content And Storage: Sufficient For: 250 colonies or 250 culture samples (100 μL each)Y-PER Yeast Protein Extraction Reagent, 25 mL (store at room temperature)2X beta-Galactosidase Assay Buffer, 25 mL (store at -20°C)1M Sodium Carbonate Stop Solution, 25 mL (store at room temperature)
For Use With (Application): Absorbance
Product Type: Yeast beta-Galactosidase Assay Kit
Format: 96-well Plate
Quantity: 75 mL
Substrate Type: Beta-Gal Substrate
Target: Beta-Galactosidase
Detection Method: Reporter Enzyme
For Use With (Equipment): Microplate Reader
Dye Type: Enzyme Labeled
Product Line: Pierce™
Substrate: ONPG
Sufficient For: 250 colonies or 250 culture samples (100 μL each)
Content And Storage:
Sufficient For: 250 colonies or 250 culture samples (100 μL each)Y-PER Yeast Protein Extraction Reagent, 25 mL (store at room temperature)2X beta-Galactosidase Assay Buffer, 25 mL (store at -20°C)1M Sodium Carbonate Stop Solution, 25 mL (store at room temperature)
For Use With (Application):
Absorbance
Product Type:
Yeast beta-Galactosidase Assay Kit
Format:
96-well Plate
Quantity:
75 mL
Substrate Type:
Beta-Gal Substrate
Target:
Beta-Galactosidase
Detection Method:
Reporter Enzyme
For Use With (Equipment):
Microplate Reader
Dye Type:
Enzyme Labeled
Product Line:
Pierce™
Substrate:
ONPG
Sufficient For:
250 colonies or 250 culture samples (100 μL each)
Content And Storage: Store membranes flat at ambient temperature, away from chemical vapors. Some solvent vapors may partially dissolve the membranes, which will disrupt the pore structure. Keep membranes out of direct sunlight. Sunlight may cause the nitrocellulose to dry and become brittle. Note: Nitrocellulose membrane filters are highly flammable. Keep away from heat and open flame. Flash point is approximately 2°C. Membranes can be sterilized by steam-autoclaving at 121°C.
For Use With (Application): __
Product Type: __
Format: Sheet
Quantity: 25 Pre-cut Membranes
Substrate Type: __
Target: __
Detection Method: __
For Use With (Equipment): __
Dye Type: __
Product Line: __
Substrate: __
Sufficient For: 25 Mini-gel Blots
Content And Storage:
Store membranes flat at ambient temperature, away from chemical vapors. Some solvent vapors may partially dissolve the membranes, which will disrupt the pore structure. Keep membranes out of direct sunlight. Sunlight may cause the nitrocellulose to dry and become brittle. Note: Nitrocellulose membrane filters are highly flammable. Keep away from heat and open flame. Flash point is approximately 2°C. Membranes can be sterilized by steam-autoclaving at 121°C.
For Use With (Application):
__
Product Type:
__
Format:
Sheet
Quantity:
25 Pre-cut Membranes
Substrate Type:
__
Target:
__
Detection Method:
__
For Use With (Equipment):
__
Dye Type:
__
Product Line:
__
Substrate:
__
Sufficient For:
25 Mini-gel Blots
Content And Storage: Store membranes flat at ambient temperature, away from chemical vapors. Some solvent vapors may partially dissolve the membranes, which will disrupt the pore structure. Keep membranes out of direct sunlight. Sunlight may cause the nitrocellulose to dry and become brittle. Note: Nitrocellulose membrane filters are highly flammable. Keep away from heat and open flame. Flash point is approximately 2°C. Membranes can be sterilized by steam-autoclaving at 121°C.
For Use With (Application): __
Product Type: __
Format: Sheet
Quantity: 25 Pre-cut Membranes
Substrate Type: __
Target: __
Detection Method: __
For Use With (Equipment): __
Dye Type: __
Product Line: __
Substrate: __
Sufficient For: 25 Mini-gel Blots
Content And Storage:
Store membranes flat at ambient temperature, away from chemical vapors. Some solvent vapors may partially dissolve the membranes, which will disrupt the pore structure. Keep membranes out of direct sunlight. Sunlight may cause the nitrocellulose to dry and become brittle. Note: Nitrocellulose membrane filters are highly flammable. Keep away from heat and open flame. Flash point is approximately 2°C. Membranes can be sterilized by steam-autoclaving at 121°C.
For Use With (Application):
__
Product Type:
__
Format:
Sheet
Quantity:
25 Pre-cut Membranes
Substrate Type:
__
Target:
__
Detection Method:
__
For Use With (Equipment):
__
Dye Type:
__
Product Line:
__
Substrate:
__
Sufficient For:
25 Mini-gel Blots
Content And Storage: Note: Always wear gloves when handling membranes because oils from fingers may prevent proper wetting. Also, proteins and other contaminants from hands may bind to the membranes and cause background.
For Use With (Application): __
Product Type: __
Format: Sheet
Quantity: 25 sheets
Substrate Type: __
Target: __
Detection Method: __
For Use With (Equipment): __
Dye Type: __
Product Line: Biodyne
Substrate: __
Sufficient For: __
Content And Storage:
Note: Always wear gloves when handling membranes because oils from fingers may prevent proper wetting. Also, proteins and other contaminants from hands may bind to the membranes and cause background.
For Use With (Application):
__
Product Type:
__
Format:
Sheet
Quantity:
25 sheets
Substrate Type:
__
Target:
__
Detection Method:
__
For Use With (Equipment):
__
Dye Type:
__
Product Line:
Biodyne
Substrate:
__
Sufficient For:
__