R37109

Molecular Probes™ NucGreen™ Dead 488 ReadyProbes™ Reagent (SYTOX™ Green)

Manufacturer: Molecular Probes™

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Pack Size SKU Availability Price
Each of 1 R37109-Each-of-1 In Stock ₹ 25,753.56

R37109 - Each of 1

₹ 25,753.56

In Stock

Quantity

1

Base Price: ₹ 25,753.56

GST (18%): ₹ 4,635.641

Total Price: ₹ 30,389.201

Cell Permeability

Cell-impermeant

Content And Storage

6 x 2.5 mL dropper bottlesStore at ≤ 25°C

For Use With (Application)

Viability Assay

Product Type

Nucleic Acid Stain

Emission

523 nm

Form

Liquid

Quantity

6 Bottles

Color

Green

Detection Method

Fluorescence

For Use With (Equipment)

Confocal Microscope, Floid Cell Imaging System, Fluorescence Microscope, Flow Cytometer

Dye Type

Other Label(s) or Dye(s)

Excitation Wavelength Range

504 nm

Product Line

NucGreen, ReadyProbes

Sub Cellular Localization

Nucleus

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Description

  • NucGreen Dead 488 ReadyProbes Reagent is a cell-impermeant stain that emits exceptionally bright, green fluorescence when bound to DNA
  • Cells that have lost plasma membrane integrity are stained within minutes, making this an extremely useful stain to estimate live/dead cell ratios and to measure cytotoxicity in kinetic live-cell assays
  • It is also suitable for staining nuclei in fixed cell preparations and tissue sections
  • • Exceptionally bright green fluorescence upon binding to DNA • Rapid staining of damaged, dead, or fixed cells without wash steps • Ready-to-use liquid formulation in convenient dropper bottle—no need to dilute, weigh, or pipette • Stability at room temperature—keep handy at your scope or cell Cell imaging applications The membrane-impermeable NucGreen Dead 488 reagent is a high-affinity DNA stain that easily stains cells with compromised cell membranes, yet does not enter living cells
  • It is therefore ideal for discrimination of live and dead cells
  • The fluorescence increases more than 500-fold on binding to dsDNA in cells with compromised cell membranes (dying or dead cells), in fixed cells, and tissue slices
  • The spectral properties, with excitation/emission at 504/523 nm when bound to DNA, are ideal for standard FITC filters
  • Suggestions for use • NucGreen Dead 488 reagent may be added directly to cells in full media or buffer solutions • It easily stains nuclei of fixed cells and tissue slices • In most cases, 2 drops/ml and an incubation time of 15 to 30 minutes will give bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications
  • In such cases, simply add more or fewer drops until the optimal staining intensity is obtained
  • In most cases, staining intensity increases with time if cells are not washed prior to imaging
  • • NucGreen Dead 488 dye is excited with a maximum at 504 nm when bound to DNA, with an emission maximum at 523 nm
  • It is detected through standard GFP and FITC filters.

Compare Similar Items

Show Difference

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Molecular Probes™

R37109

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Cell Permeability:
Cell-impermeant

Content And Storage:
6 x 2.5 mL dropper bottlesStore at ≤ 25°C

For Use With (Application):
Viability Assay

Product Type:
Nucleic Acid Stain

Emission:
523 nm

Form:
Liquid

Quantity:
6 Bottles

Color:
Green

Detection Method:
Fluorescence

For Use With (Equipment):
Confocal Microscope, Floid Cell Imaging System, Fluorescence Microscope, Flow Cytometer

Dye Type:
Other Label(s) or Dye(s)

Excitation Wavelength Range:
504 nm

Product Line:
NucGreen, ReadyProbes

Sub Cellular Localization:
Nucleus

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Cell Permeability:
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Content And Storage:
2 × 2.5 mL dropper bottlesStore at ≤ 25°C.

For Use With (Application):
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Product Type:
Phalloidin

Emission:
Visible

Form:
Liquid

Quantity:
1 kit

Color:
Green

Detection Method:
Fluorescence

For Use With (Equipment):
Confocal Microscope, Floid™ Cell Imaging System, Fluorescence Microscope, Flow Cytometer, Floid™ Cell Imaging System

Dye Type:
Alexa Fluor™ 488

Excitation Wavelength Range:
495⁄518

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Sub Cellular Localization:
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Content And Storage:
1 × 1.75 mL dropper bottlesStore at ≤ 25°C.

For Use With (Application):
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Emission:
__

Form:
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Quantity:
1.75 mL

Color:
Green

Detection Method:
Fluorescence

For Use With (Equipment):
Confocal Microscope, Floid™ Cell Imaging System, Fluorescence Microscope, Flow Cytometer, Floid™ Cell Imaging System

Dye Type:
__

Excitation Wavelength Range:
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CellEvent™, ReadyProbes™

Sub Cellular Localization:
__

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Cell Permeability:
__

Content And Storage:
2 × 2.5 mL dropper bottlesStore at ≤ 25°C.

For Use With (Application):
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Product Type:
Phalloidin

Emission:
Visible

Form:
Liquid

Quantity:
2 dropper bottle(s)

Color:
Red-Orange

Detection Method:
Fluorescence

For Use With (Equipment):
Confocal Microscope, EVOS™, Floid™ Cell Imaging System, Fluorescence Microscope, Flow Cytometer, Floid™ Cell Imaging System

Dye Type:
TRITC (Tetramethylrhodamine Isothiocyanate)

Excitation Wavelength Range:
540⁄565

Product Line:
ActinRed™, ReadyProbes™

Sub Cellular Localization:
__