Invitrogen™ SBFI, Tetraammonium Salt, cell impermeant

Description

• Useful in combination with other fluorescent indicators used to correlate changes in intracellular Na + with Ca 2+ and Mg 2+ concentrations, intracellular pH, and membrane potential Although selectivity of SBFI for Na + is less than that of calcium indicators such as fura-2, it is sufficient for detection of physiological concentrations of Na + in presence of other monovalent cations The spectral response of SBFI upon ion binding permit excitation ratio measurements, and this indicator can be used with same optical filters and equipment used for fura-2 Label (Ex/Em): SBFI (approximately 340,380/500nm) Lyophilized product may be dissolved in distilled water or aqueous buffer for use Product is typically loaded into cells by diffusion from patch pipet for correlated fluorescence imaging and electrophysiological recording Dissociation constant (K d ) of SBFI for Na + is 3.8mm in the absence of K + , and 11.3mm in solutions with combined Na + and K + concentration of 135mm (which approximates physiological ionic strength)
• SBFI is approximately 18-fold more selective for Na + than for K + Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling, Sodium and Potassium Detection Order Info Shipping Condition: Room temperature